Rüter J, Raczek D I
Zentrale Analytik Bayer AG, Leverkusen, Bundesrepublik Deutschland.
Z Lebensm Unters Forsch. 1992 Jun;194(6):520-3. doi: 10.1007/BF01185475.
A sensitive and selective high pressure liquid chromatography (HPLC) procedure for the determination of sodium cyclamate in juices and preserves is presented. The method depends on the oxidation of cyclamate to cyclohexylamine, which then is converted prechromatographically into a fluorescent derivative. It is analyzed by HPLC on a C18:reversed-phase column and determined with fluorescence detection (excitation at 350 nm, emission at 440-650 nm). The detection limit of sodium cyclamate was 0.5-5 mg/kg, depending on the nature and dilution of the samples. The relative standard deviations thus obtained were +/- 1.0 to +/- 2.6%. The average recovery was 90%.
本文介绍了一种灵敏且具选择性的高效液相色谱（HPLC）方法，用于测定果汁和果酱中的甜蜜素钠。该方法基于甜蜜素氧化为环己胺，然后在色谱分离前将其转化为荧光衍生物。通过在C18反相柱上进行HPLC分析，并采用荧光检测（激发波长350nm，发射波长440 - 650nm）进行测定。甜蜜素钠的检测限为0.5 - 5mg/kg，具体取决于样品的性质和稀释度。由此获得的相对标准偏差为±1.0%至±2.6%。平均回收率为90%。
