Ohtake Satoshi, Schebor Carolina, Palecek Sean P, de Pablo Juan J
Department of Chemical and Biological Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, WI 53706, USA.
Cryobiology. 2004 Feb;48(1):81-9. doi: 10.1016/j.cryobiol.2004.01.001.
The stabilizing role of sugars on dehydrated membranes is well established. The formation of a glassy matrix and the direct interaction between the sugars and the lipids are some of the mechanisms proposed to be involved in this stabilizing effect. Phospholipidic systems have been studied extensively as models for biological membranes and also due to the practical applications of liposomes as vehicles for drug delivery. In this work, we evaluate the effect of sugar-phosphate mixtures on the transition temperature of dehydrated 1,2-dipalmitoylphosphatidylcholine, and also examine some physical characteristics of these mixtures, such as the glass transition temperature and water sorption properties. The addition of phosphate salts to sugar systems has several interesting features that merit its consideration in formulations to protect dehydrated labile biomaterials. In particular, sucrose-phosphate mixtures provide an interesting alternative to pure saccharide formulations due to their high glass transition temperatures and their increased ability to maintain a low melting transition temperature in the presence of small amounts of water.
糖类对脱水膜的稳定作用已得到充分证实。玻璃态基质的形成以及糖类与脂质之间的直接相互作用是被认为参与这种稳定作用的一些机制。磷脂体系作为生物膜的模型已被广泛研究，同时也因其作为药物递送载体的实际应用而受到关注。在这项工作中，我们评估了糖 - 磷酸盐混合物对脱水的1,2 - 二棕榈酰磷脂酰胆碱转变温度的影响，并研究了这些混合物的一些物理特性，如玻璃化转变温度和吸水性能。向糖类体系中添加磷酸盐盐具有几个有趣的特性，这使其在用于保护脱水的易损生物材料的配方中值得考虑。特别是，蔗糖 - 磷酸盐混合物因其高玻璃化转变温度以及在存在少量水的情况下保持低熔点转变温度的能力增强，为纯糖类配方提供了一个有趣的替代方案。
