Varella-Garcia Marileila, Akduman Bulent, Sunpaweravong Patrapim, Di Maria Michael V, Crawford E David
University of Colorado Comprehensive Cancer Center, UCHSC, Denver CO, USA.
Urol Oncol. 2004 Jan-Feb;22(1):16-9. doi: 10.1016/S1078-1439(03)00098-X.
The newly developed UroVysion fluorescence in situ hybridization (FISH) probe was applied to urine specimens from 19 patients being monitored for recurrence of bladder cancer. The results for the multi-target DNA FISH assay were compared with independent analyses of urine cytology and flexible cystoscopy. Patients with tumors identified through the cystoscopy exam were biopsied and/or underwent surgery. In 12 patients with normal cytoscopy, cytology and FISH were also normal. Therefore, the specificity of these two tests was 100%. In 7 patients, a tumor was diagnosed by cystoscopy, and 3 of them had abnormal urine cytology while 6 of them had an abnormal result in the FISH assay. Accordingly, the sensitivity was 43% for the cytology and 87% for the FISH test. Interestingly, a pT1G3 tumor in a bladder diverticulum was not detected by cytology or the FISH test. These results agreed with a large series previously published using similar FISH probes and support the proposal for a multicenter trial to confirm the usefulness of the UroVysion probe as a screening tool to select patients for cystoscopy.
将新开发的UroVysion荧光原位杂交（FISH）探针应用于19例膀胱癌复发监测患者的尿液标本。将多靶点DNA FISH检测结果与尿液细胞学和软性膀胱镜检查的独立分析结果进行比较。通过膀胱镜检查发现肿瘤的患者接受了活检和/或手术。在12例膀胱镜检查正常的患者中，细胞学和FISH检查也均正常。因此，这两项检查的特异性均为100%。在7例患者中，膀胱镜检查诊断为肿瘤，其中3例尿液细胞学检查异常，6例FISH检测结果异常。因此，细胞学检查的敏感性为43%，FISH检测的敏感性为87%。有趣的是，膀胱憩室内的pT1G3肿瘤未被细胞学检查或FISH检测发现。这些结果与之前使用类似FISH探针发表的大量研究结果一致，并支持进行多中心试验以证实UroVysion探针作为筛选工具选择患者进行膀胱镜检查的有用性的提议。
