Yang Dong-Hua, Huang Wei, Cui Jun, Shu Jian-Chang, Tang Shao-Hui, Zhang Wen-Jie, Liang Jie-Hua
Department of Gastroenterology, First Affiliated Hospital, Jinan University, Guangzhou 510630, China.
Hepatobiliary Pancreat Dis Int. 2004 Feb;3(1):86-9.
Recent research found abnormal expression of the c-fms oncogene, which encodes the macrophage colony-stimulating factor receptor (CSF-1R), in several human carcinomas including hepatocellular carcinoma (HCC). But the relationship between the point mutation and abnormal expressing of c-fms oncogene in HCC was not clear. This study is to investigate the relationship between point mutation and abnormal expression of c-fms oncogene in hepatocellular carcinoma (HCC) and to clarify the mechanism of HCC.
The expression of c-fms oncogene at different levels of cell, protein and transcription was observed using immune histological ABC, Western blot and Northern blot. PCR-single strand conformation polymorphism and gene sequencing were used to detect the mutation of c-fms in HCC tissues and their surrounding tissues of 30 patients.
The expression of c-fms was significantly higher in HCC tissues than in their surrounding tissues (P<0.01). Point mutation of Leu (TTG)-->Ser (TCG) at codon 301 of c-fms amino acids was observed in 21.4% (3/14) HCC tissues. No mutation of c-fms oncogene was detected in the surrounding cancerous tissues.
Point mutation at codon 301 of c-fms oncogene is one of the mechanisms of abnormal over-expression in HCC.
最近的研究发现，编码巨噬细胞集落刺激因子受体（CSF-1R）的c-fms癌基因在包括肝细胞癌（HCC）在内的几种人类癌症中表达异常。但HCC中c-fms癌基因的点突变与异常表达之间的关系尚不清楚。本研究旨在探讨肝细胞癌（HCC）中c-fms癌基因的点突变与异常表达之间的关系，并阐明HCC的发病机制。
采用免疫组织化学ABC法、蛋白质印迹法和Northern印迹法观察c-fms癌基因在细胞、蛋白质和转录水平的表达。应用聚合酶链反应-单链构象多态性（PCR-SSCP）和基因测序技术检测30例HCC患者癌组织及其癌旁组织中c-fms基因的突变情况。
HCC组织中c-fms的表达明显高于其癌旁组织（P<0.01）。在21.4%（3/14）的HCC组织中观察到c-fms氨基酸第301密码子处发生Leu（TTG）→Ser（TCG）的点突变。癌旁组织中未检测到c-fms癌基因的突变。
c-fms癌基因第301密码子处的点突变是HCC异常过表达的机制之一。
