Grulke N E, Hom J L, Roberts S W
Forest Service, U.S. Department of Agriculture, Pacific Southwest Forest and Range Experiment Station, 4955 Canyon Crest Drive, Riverside, CA 92507, USA.
Tree Physiol. 1993 Jun;12(4):391-401. doi: 10.1093/treephys/12.4.391.
Seeds from two full-sib families of ponderosa pine (Pinus ponderosa) with known differences in growth rates were germinated and grown in an ambient (350 micro l l(-1)) or elevated (700 micro l l(-1)) CO(2) concentration. Gas exchange at both ambient and elevated CO(2) concentrations was measured 1, 6, 39, and 112 days after the seed coat was shed. Initial stimulation of CO(2) exchange rate (CER) by elevated CO(2) was large (> 100%). On Day 1, CER of seedlings grown in elevated CO(2) and measured at ambient CO(2) was significantly lower than the CER of seedlings grown and measured at ambient CO(2), indicating physiological adjustment of the seedlings exposed to elevated CO(2). Physiological acclimation to elevated CO(2) was complete by Day 39 when there was no significant difference in CER between seedlings grown and measured at ambient CO(2) and seedlings grown and measured at elevated CO(2). After 4 months, the light response of seedlings in the two treatments was determined at both ambient and elevated CO(2). Light compensation point, CER at light saturation, and apparent quantum efficiency of seedlings grown and measured at ambient CO(2) were not significantly different from those of seedlings grown and measured at elevated CO(2). With a short-term increase in CO(2), CER at light saturation (5.16 +/- 0.52 versus 3.13 +/- 0.30 micro mol CO(2) m(-2) s(-1)) and apparent quantum efficiency (0.082 +/- 0.011 versus 0.045 +/- 0.003 micro mol CO(2) micro mol(-1) quanta) were significantly increased. Leaf C/N ratio was significantly increased in the elevated CO(2) treatment. There were few significant differences between families for any response to elevated CO(2). Under the experimental conditions, high growth rate was not correlated with a greater response to elevated CO(2).
从两个已知生长速率存在差异的黄松（Pinus ponderosa）全同胞家系中获取种子，将其在环境二氧化碳浓度（350 μl l⁻¹）或升高的二氧化碳浓度（700 μl l⁻¹）下进行萌发和培育。在种皮脱落1、6、39和112天后，测定环境二氧化碳浓度和升高的二氧化碳浓度下的气体交换情况。升高的二氧化碳对二氧化碳交换速率（CER）的初始刺激作用很大（>100%）。在第1天，在升高的二氧化碳浓度下生长并在环境二氧化碳浓度下测定的幼苗的CER显著低于在环境二氧化碳浓度下生长并测定的幼苗的CER，这表明暴露于升高的二氧化碳浓度下的幼苗发生了生理调节。到第39天，对升高的二氧化碳浓度的生理适应完成，此时在环境二氧化碳浓度下生长并测定的幼苗与在升高的二氧化碳浓度下生长并测定的幼苗的CER没有显著差异。4个月后，在环境二氧化碳浓度和升高的二氧化碳浓度下测定了两种处理中幼苗的光响应。在环境二氧化碳浓度下生长并测定的幼苗的光补偿点、光饱和时的CER和表观量子效率与在升高的二氧化碳浓度下生长并测定的幼苗没有显著差异。随着二氧化碳的短期增加，光饱和时的CER（5.16±0.52对3.13±0.30 μmol CO₂ m⁻² s⁻¹）和表观量子效率（0.082±0.011对0.045±0.003 μmol CO₂ μmol⁻¹量子）显著增加。在升高的二氧化碳浓度处理中，叶片碳氮比显著增加。对于升高的二氧化碳浓度的任何响应，家系之间几乎没有显著差异。在实验条件下，高生长速率与对升高的二氧化碳浓度的更大响应无关。
