基于细菌人工染色体支架图、全基因组辐射杂种细胞系和人类草图序列绘制的牛19号染色体比较图谱。

A comparative map of bovine chromosome 19 based on a combination of mapping on a bacterial artificial chromosome scaffold map, a whole genome radiation hybrid panel and the human draft sequence.

作者信息

Moore S S, Hansen C, Williams J L, Fu A, Meng Y, Li C, Zhang Y, Urquhart B S D, Marra M, Schein J, Benkel B, de Jong P J, Osoegawa K, Kirkpatrick B W, Gill C A

机构信息

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.

出版信息

Cytogenet Genome Res. 2003;102(1-4):32-8. doi: 10.1159/000075721.

DOI:10.1159/000075721

PMID:14970675

Abstract

We have constructed a medium density physical map of bovine chromosome 19 using a combination of mapping loci on both a bovine bacterial artificial chromosome (BAC) scaffold map and a whole genome radiation hybrid (WGRH) panel. The resulting map contains 70 loci spanning the length of bovine chromosome 19. Three contiguous groups of BACs were identified on the basis of multiple loci mapping to individual BAC clones. Bovine chromosome 19 was found in this study to be comprised almost entirely from regions of human chromosome 17, with a small region putatively assigned to human chromosome 10. Fourteen breakpoints between the bovine and human chromosomes were detected, with a possibility of five more based on ordering of the WGRH map.

摘要

我们使用牛细菌人工染色体（BAC）支架图上的定位位点与全基因组辐射杂种（WGRH）板相结合的方法，构建了牛19号染色体的中等密度物理图谱。所得图谱包含跨越牛19号染色体全长的70个位点。基于多个位点映射到单个BAC克隆，鉴定出了三个连续的BAC组。在本研究中发现，牛19号染色体几乎完全由人类17号染色体区域组成，有一个小区域可能归属于人类10号染色体。检测到牛和人类染色体之间有14个断点，根据WGRH图谱的排序，可能还有5个断点。