Bosak N, Faraut T, Mikawa S, Uenishi H, Kiuchi S, Hiraiwa H, Hayashi T, Yasue H
Genome Research Department, National Institute of Agrobiological Sciences, Ikenodai, Tsukuba, Ibaraki, Japan.
Cytogenet Genome Res. 2003;102(1-4):109-15. doi: 10.1159/000075734.
A comprehensive and comparative map was constructed for the porcine chromosome (SSC) 6q11-->q21 region, where the gene(s) responsible for the maldevelopment of embryos are localized using swine populations of the National Institute of Animal Industry, Japan (NIAI). Since the chromosomal region corresponds to a region of human chromosome (HSA) 19q13.1-->q13.3 based on bi-directional chromosome painting, primer pairs were designed from porcine cDNA sequences identified, on a sequence comparison basis, as being transcripts from genes orthologous to those in the HSA region. Fifty-one genes were successfully assigned to a swine radiation hybrid (RH) map with LOD scores greater than 6. ERF and PSMD8 genes were assigned to SSC4 and SSC1, respectively. The remaining 49 genes were assigned to SSC6, demonstrating that the synteny between the SSC6 and HSA19 chromosomal regions is essentially conserved, therefore confirming, the results of bi-directional chromosome painting. However, when examined precisely, rearrangements have apparently occurred within the region of conserved synteny. For the ERF and PSMD8 genes assigned to SSCs other than SSC6, additional mapping using somatic cell hybrid (SCH) panels was performed to confirm the results of RH-mapping.
利用日本国立动物产业研究所（NIAI）的猪群构建了猪6号染色体（SSC）6q11→q21区域的综合比较图谱，该区域定位了导致胚胎发育不良的基因。基于双向染色体涂染，该染色体区域对应于人类染色体（HSA）19q13.1→q13.3区域，因此根据序列比较，从猪cDNA序列中设计了引物对，这些序列被鉴定为与HSA区域基因直系同源的转录本。51个基因成功定位到猪辐射杂种（RH）图谱上，LOD得分大于6。ERF和PSMD8基因分别定位到SSC4和SSC1上。其余49个基因定位到SSC6上，表明SSC6和HSA19染色体区域之间的同线性基本保守，从而证实了双向染色体涂染的结果。然而，仔细检查时，在保守同线性区域内显然发生了重排。对于定位到SSC6以外的SSC上的ERF和PSMD8基因，使用体细胞杂种（SCH）板进行了额外的定位，以确认RH定位的结果。
