DeSilva U, Franklin I R, Maddox J F, van Hest B, Adelson D L
CSIRO Livestock Industries, Indooroopilly, QLD, Australia.
Cytogenet Genome Res. 2003;102(1-4):79-84. doi: 10.1159/000075729.
65,000 sheep skin cDNA clones were gridded in high density on to nylon membranes and screened for (CA)n and (GA)n repeat containing clones. 296 dinucleotide repeat-containing clones were identified with approximately 85% non-redundancy. Clones were single-pass 5' sequenced and we compared the Expressed Sequence Tag (EST) sequences to the Swiss-Prot database to ascertain their identity and/or putative function. We then aligned the ESTs against the human genomic sequence to determine the locations of human orthologous sequences. Finally, we developed a subset of polymorphic microsatellite markers and positioned them on the ovine linkage map.
将65000个绵羊皮肤cDNA克隆高密度点阵到尼龙膜上，筛选含有（CA）n和（GA）n重复序列的克隆。鉴定出296个含二核苷酸重复序列的克隆，其冗余度约为85%。对克隆进行单通道5'端测序，并将表达序列标签（EST）序列与瑞士蛋白质数据库进行比较，以确定其身份和/或推定功能。然后将EST与人类基因组序列进行比对，以确定人类直系同源序列的位置。最后，开发了一组多态性微卫星标记，并将其定位到绵羊连锁图谱上。
