Panangala V S, Morsy M A, Gresham M M, Toivio-Kinnucan M
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5516.
Am J Vet Res. 1992 Jul;53(7):1139-44.
A panel of monoclonal antibodies (MAb) developed against Mycoplasma gallisepticum strain PG31 was used to probe the antigenic profiles of 5 recognized strains (PG31, R, S6, F, A5969) and 6 field isolates of M gallisepticum. Monoclonal antibody G9 predominantly recognized antigens at apparent molecular mass positions of 90 to 98 kDA. The MAb reacted with all strains and isolates, but the molecular mass position of the antigens varied among some mycoplasmas. Monoclonal antibody G12 reacted with all strains and isolates of M gallisepticum and had an identical banding pattern. However, MAb G10 and G11 reacted selectively only with a limited number of strains and/or isolates. Surface distribution of the MAb-recognized antigens was revealed by immunoelectron microscopy. Partial physicochemical characterization of MAb G9-recognized antigens identified glycopeptide characteristics. Monoclonal antibody G9 reacted with surface antigens and, hence, participated in agglutination of M gallisepticum. However, the degree of agglutination varied among the various strains and isolates, indicating a quantitative or conformational limitation or an alteration in the anomeric expression of the epitopes. Antigenic variation in M gallisepticum may be mediated by immunologic selective pressures, or a proclivity for habit niche in the host.
一组针对鸡毒支原体PG31株研制的单克隆抗体（MAb）被用于检测5种已知菌株（PG31、R、S6、F、A5969）和6株鸡毒支原体田间分离株的抗原谱。单克隆抗体G9主要识别表观分子量在90至98 kDa的抗原。该单克隆抗体与所有菌株和分离株都发生反应，但不同支原体之间抗原的分子量位置有所不同。单克隆抗体G12与所有鸡毒支原体菌株和分离株都发生反应，且具有相同的条带模式。然而，单克隆抗体G10和G11仅选择性地与有限数量的菌株和/或分离株发生反应。通过免疫电子显微镜揭示了单克隆抗体识别抗原的表面分布。对单克隆抗体G9识别抗原的部分理化特性鉴定表明其具有糖肽特征。单克隆抗体G9与表面抗原发生反应，因此参与了鸡毒支原体的凝集反应。然而，不同菌株和分离株之间的凝集程度有所不同，这表明存在定量或构象限制，或者表位的异头表达发生了改变。鸡毒支原体的抗原变异可能由免疫选择压力介导，或者是宿主中偏好的栖息生态位所致。
