Liu L, Payne D M, van Santen V L, Dybvig K, Panangala V S
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849, USA.
Infect Immun. 1998 Nov;66(11):5570-5. doi: 10.1128/IAI.66.11.5570-5575.1998.
A 62-kDa cell surface antigen (M9) of Mycoplasma gallisepticum PG31 that mediates antibody-induced agglutination of the organism was purified and subjected to N-terminal amino-acid sequencing. A 999-bp region of the cDNA encoding the M9 protein was generated by reverse transcription-PCR, and its nucleotide sequence was determined. PCR primers based on this sequence were used to screen a genomic DNA library of PG31. A full-length M9 protein-encoding gene was isolated and sequenced, revealing 96% nucleotide identity with the pMGA1.1 gene of M. gallisepticum S6. Sequence analyses of the M9 gene and flanking open reading frames that encode other pMGA family members suggest that a tandemly repeated GAA sequence may influence pMGA gene expression.
鸡毒支原体PG31的一种介导抗体诱导的菌体凝集的62 kDa细胞表面抗原（M9）被纯化，并进行了N端氨基酸测序。通过逆转录PCR产生了编码M9蛋白的cDNA的999 bp区域，并确定了其核苷酸序列。基于该序列的PCR引物用于筛选PG31的基因组DNA文库。分离并测序了全长M9蛋白编码基因，发现与鸡毒支原体S6的pMGA1.1基因有96%的核苷酸同一性。M9基因和编码其他pMGA家族成员的侧翼开放阅读框的序列分析表明，串联重复的GAA序列可能影响pMGA基因表达。
