使用组合甲基化特异性PCR分析检测前列腺分泌物中的GSTP1甲基化

Detection of GSTP1 methylation in prostatic secretions using combinatorial MSP analysis.

作者信息

Gonzalgo Mark L, Nakayama Masashi, Lee Shing M, De Marzo Angelo M, Nelson William G

机构信息

Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.

出版信息

Urology. 2004 Feb;63(2):414-8. doi: 10.1016/j.urology.2003.08.039.

DOI:10.1016/j.urology.2003.08.039

PMID:14972513

Abstract

OBJECTIVES

To evaluate the utility of methylation-specific polymerase chain reaction analysis of the pi-class glutathione-S-transferase (GSTP1) gene promoter in prostatic secretions for cancer detection and prognostication.

METHODS

Prostatic secretions were obtained from a total of 100 radical prostatectomy specimens immediately after surgical extirpation. GSTP1 promoter methylation was assessed by methylation-specific polymerase chain reaction analysis using two different primer sets. Correlations between GSTP1 promoter methylation and clinical and pathologic variables were examined.

RESULTS

The sensitivity for detection of GSTP1 methylation in prostatic secretions from men with clinically localized prostate cancer using two different primer sets was 76% and 54%. Methylation of the GSTP1 promoter was detected by both primer sets in 44% and by at least one primer set in 86% of the prostatic secretion specimens. The degree of methylation detected in the prostatic secretions was associated with the extent of cancer (predominant involvement of one or both sides of the gland; P = 0.02) and increasing age (P = 0.009).

CONCLUSIONS

Genomic DNA with GSTP1 promoter methylation can be detected in prostatic secretion specimens from the great majority of men with localized prostate cancer. Assays of GSTP1 promoter methylation in prostatic massage fluid or ejaculate may therefore serve as useful adjuncts to existing methods for prostate cancer screening and prognostication.

摘要

目的

评估前列腺分泌物中π类谷胱甘肽-S-转移酶（GSTP1）基因启动子的甲基化特异性聚合酶链反应分析在前列腺癌检测和预后评估中的效用。

方法

在手术切除后立即从总共100份前列腺癌根治术标本中获取前列腺分泌物。使用两种不同的引物组通过甲基化特异性聚合酶链反应分析评估GSTP1启动子甲基化。检查GSTP1启动子甲基化与临床和病理变量之间的相关性。

结果

使用两种不同引物组检测临床局限性前列腺癌男性前列腺分泌物中GSTP1甲基化的灵敏度分别为76%和54%。在44%的前列腺分泌物标本中，两种引物组均检测到GSTP1启动子甲基化，在86%的标本中至少一种引物组检测到甲基化。在前列腺分泌物中检测到的甲基化程度与癌症范围（腺体一侧或两侧的主要受累情况；P = 0.02）和年龄增长（P = 0.009）相关。