猫泡沫病毒反式激活因子的比较功能特性揭示了其物种特异性。

Comparative functional characterization of the feline foamy virus transactivator reveals its species specificity.

作者信息

Bodem Jochen, Kang Yibin, Flügel Rolf M

机构信息

Retroviral Gene Expression, German Cancer Research Center, Applied Tumorvirology, D-69009 Heidelberg, Germany.

出版信息

Virology. 2004 Jan 5;318(1):32-6. doi: 10.1016/j.virol.2003.09.039.

DOI:10.1016/j.virol.2003.09.039

PMID:14972532

Abstract

Foamy virus (FV) Bel1/Tas transactivators act as key regulators of gene expression and directly bind DNA Bel1 response elements (BREs) in both the internal (IP) and 5'LTR promoters. Here, we report the mapping and the virus species specificity of the nonhomologous feline foamy virus (FFV) BREs in both promoters. The data indicate that FFV Bel1 did not bind the primate FV IP.BRE and that primate FV Bel1 was not capable of binding the FFV IP.BRE. In addition, we show that the C-terminal activation domain of FFV Bel1 does not contribute to DNA binding because a C-terminal trans-dominant negative FFV Bel1 mutant was still able to bind to both promoters.

摘要

泡沫病毒（FV）的Bel1/Tas反式激活因子作为基因表达的关键调节因子，直接结合内部（IP）启动子和5'LTR启动子中的DNA Bel1反应元件（BRE）。在此，我们报告了两个启动子中非同源猫泡沫病毒（FFV）BRE的定位及病毒种属特异性。数据表明，FFV Bel1不结合灵长类FV的IP.BRE，灵长类FV Bel1也不能结合FFV的IP.BRE。此外，我们发现FFV Bel1的C端激活结构域对DNA结合没有贡献，因为C端反式显性负性FFV Bel1突变体仍能结合两个启动子。