State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, Nankai University, Tianjin 300071, China.
Virology. 2010 Sep 30;405(2):408-13. doi: 10.1016/j.virol.2010.05.037. Epub 2010 Jul 7.
The genome of bovine foamy virus (BFV) encodes a transcriptional transactivator, namely BTas, that remarkably enhances gene expression by binding to the viral long-terminal repeat promoter (LTR) and internal promoter (IP). In this report, we characterized the functional domains of BFV BTas. BTas contains two major functional domains: the N-terminal DNA-binding domain (residues 1-133) and the C-terminal activation domain (residues 198-249). The complete BTas responsive regions were mapped to the positions -380/-140 of LTR and 9205/9276 of IP. Four BTas responsive elements were identified at the positions -368/-346, -327/-307, -306/-285 and -186/-165 of the BFV LTR, and one element was identified at the position 9243/9264 of the BFV IP. Unlike other foamy viruses, the five BTas responsive elements in BFV shared obvious sequence homology. These data suggest that among the complex retroviruses, BFV appears to have a unique transactivation mechanism.
牛泡沫病毒（BFV）的基因组编码一种转录反式激活物，即 BTas，它通过与病毒长末端重复启动子（LTR）和内部启动子（IP）结合，显著增强基因表达。在本报告中，我们对 BFV BTas 的功能结构域进行了特征描述。BTas 包含两个主要的功能结构域：N 端 DNA 结合结构域（残基 1-133）和 C 端激活结构域（残基 198-249）。完整的 BTas 反应区域被映射到 LTR 的-380/-140 位置和 IP 的 9205/9276 位置。在 BFV LTR 的-368/-346、-327/-307、-306/-285 和-186/-165 位置鉴定出四个 BTas 反应元件，在 BFV IP 的 9243/9264 位置鉴定出一个元件。与其他泡沫病毒不同，BFV 中的五个 BTas 反应元件具有明显的序列同源性。这些数据表明，在复杂的逆转录病毒中，BFV 似乎具有独特的转录激活机制。
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