Miller S L, Durall D M, Rygiewicz P T
Department of Forest Science, Oregon State University, Corvallis, OR 97331, USA.
Tree Physiol. 1989 Jun;5(2):239-49. doi: 10.1093/treephys/5.2.239.
Ponderosa pine seedlings were inoculated with Hebeloma crustuliniforme either in growth pouches before they were transplanted to root-mycocosms (P seedlings), or at the time of transfer to root-mycocosms (V seedlings). Uninoculated seedlings served as controls (U seedlings). The use of root-mycocosms allowed examination of portions of hyphae separate from roots and rooting substrate but still in symbiosis with the host. The results thus provided a quantitative basis for estimating hyphal mass and carbon allocation to extramatrical hyphae. The amount of (14)CO(2) fixed after a 2-h exposure was greatest for P seedlings and least for uninoculated seedlings. Four and nine days after exposure, (14)C content was greatest in uninoculated seedlings and least in inoculated seedlings. In isotope distribution and dry mass accumulation, V seedlings were more similar to U than to P seedlings. Calculated on a dry weight basis, the allocation of isotope to mycelium suggested that extramatrical hyphae of P seedlings were a stronger sink for carbon than extramatrical hyphae of V seedlings. Differences in inoculation methods resulted in differences in carbon allocation and physiology of extramatrical hyphae that could affect seedling establishment and survival. Seedlings inoculated by one method cannot serve as surrogates for mycorrhizal seedlings produced by other inoculation techniques.
在移植到根菌根箱之前，在生长袋中用皮壳疣柄牛肝菌接种美国黄松幼苗（P幼苗），或者在转移到根菌根箱时接种（V幼苗）。未接种的幼苗作为对照（U幼苗）。使用根菌根箱可以检查与根和生根基质分离但仍与宿主共生的部分菌丝。因此，这些结果为估计菌丝体质量和向根外菌丝的碳分配提供了定量依据。暴露2小时后固定的（14）CO（2）量，P幼苗最多，未接种幼苗最少。暴露4天和9天后，未接种幼苗的（14）C含量最高，接种幼苗最低。在同位素分布和干物质积累方面，V幼苗与U幼苗比与P幼苗更相似。以干重为基础计算，同位素向菌丝体的分配表明，P幼苗的根外菌丝比V幼苗的根外菌丝是更强的碳汇。接种方法的差异导致根外菌丝的碳分配和生理差异，这可能影响幼苗的建立和存活。通过一种方法接种的幼苗不能替代通过其他接种技术产生的菌根幼苗。
