Doumas P, Bonnet-Masimbert M, Zaerr J B
INRA, Centre de Recherches d'Orléans, Station d'amelioration des Arbres Forestiers, Ardon 45160 Olivet, France.
Tree Physiol. 1989 Mar;5(1):63-72. doi: 10.1093/treephys/5.1.63.
Concentrations of cytokinin-like compounds were measured in root tips of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) collected in spring. Cytokinin bases and ribosides were estimated by radioimmunoassay after purification by immunoaffinity (zeatin riboside and isopentenyladenosine antibodies coupled to cellulose gel) and high performance liquid chromatography. Cytokinin glucosides were similarly analyzed in root extracts treated with beta-glucosidase and octadecyl-silica column chromatography in conjunction with immunoaffinity purification. The identity of predominant cytokinins obtained after enzymatic degradation was confirmed by gas chromatography-mass spectrometry. Each of the observed cytokinin bases and ribosides seemed to have a corresponding hexose conjugate form. Total quantity of cytokinin glucosides was twice that of cytokinin bases and ribosides, and the major compound was isopentenyladenosine hexose conjugate.
测定了春季采集的花旗松（Pseudotsuga menziesii (Mirb.) Franco）根尖中细胞分裂素类化合物的浓度。通过免疫亲和（与纤维素凝胶偶联的玉米素核苷和异戊烯基腺苷抗体）和高效液相色谱纯化后，采用放射免疫分析法对细胞分裂素碱基和核苷进行了估算。用β-葡萄糖苷酶处理根提取物，并结合免疫亲和纯化，通过十八烷基硅胶柱色谱法对细胞分裂素糖苷进行了类似分析。通过气相色谱-质谱联用仪确认了酶解后得到的主要细胞分裂素的身份。观察到的每种细胞分裂素碱基和核苷似乎都有一种相应的己糖共轭形式。细胞分裂素糖苷的总量是细胞分裂素碱基和核苷总量的两倍，主要化合物是异戊烯基腺苷己糖共轭物。
