Lim Mi-Hye, Kim Joonki, Kim Youn-Sung, Chung Kyung-Sook, Seo Yeon-Hee, Lee Ilha, Kim Jungmook, Hong Choo Bong, Kim Hie-Joon, Park Chung-Mo
Graduate School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, Korea.
Plant Cell. 2004 Mar;16(3):731-40. doi: 10.1105/tpc.019331. Epub 2004 Feb 18.
Posttranscriptional RNA metabolism plays versatile roles in the regulation of gene expression during eukaryotic growth and development. It is mediated by a group of RNA binding proteins with distinct conserved motifs. In this study, an Arabidopsis (Arabidopsis thaliana) gene, designated FLK, was identified and shown to encode a putative RNA binding protein with K homology motifs. A mutant in which FLK was inactivated by T-DNA insertion exhibited a severe late flowering phenotype both in long and short days. The late flowering phenotype was reversed by gibberellin and vernalization treatments. The FLOWERING LOCUS C (FLC) transcription was greatly upregulated, whereas those of FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 decreased in the mutant. These observations demonstrate that FLK regulates the autonomous flowering pathway via FLC. It is now evident that a battery of different RNA binding proteins are involved in the posttranscriptional regulation of flowering time in Arabidopsis.
转录后RNA代谢在真核生物生长和发育过程中的基因表达调控中发挥着多种作用。它由一组具有不同保守基序的RNA结合蛋白介导。在本研究中，鉴定了一个拟南芥基因，命名为FLK，它编码一个具有K同源基序的假定RNA结合蛋白。通过T-DNA插入使FLK失活的突变体在长日照和短日照条件下均表现出严重的晚花表型。赤霉素和春化处理可逆转晚花表型。在突变体中，开花位点C（FLC）的转录大幅上调，而开花位点T和CONSTANS过表达抑制因子1的转录则下降。这些观察结果表明，FLK通过FLC调节自主开花途径。现在很明显，一系列不同的RNA结合蛋白参与了拟南芥开花时间的转录后调控。
