Suto Jun-ichi, Sekikawa Kenji
Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Biochem Genet. 2003 Oct;41(9-10):325-41. doi: 10.1023/b:bigi.0000006033.99978.8b.
A previous quantitative trait locus (QTL) study on hyperlipidemia in C57BL/6J x KK-Ay/a F2 mice identified three significant cholesterol QTLs (Cq1 and Cq2 on chromosome 1, and Cq3 on chromosome 3), and a suggestive triglyceride QTL on chromosome 9. An alternative analysis of this study identified a novel cholesterol QTL on chromosome 9 (Cq4), and a significant triglyceride QTL on chromosome 9 (Tgq1). In the present study, QTL analysis was performed on KK x RR F2 mice. A significant cholesterol QTL (Cq5, lod score 5.6) was identified on chromosome 9, and a significant triglyceride QTL (Tgq2, lod score 4.7) was identified on chromosome 8. The Cq5 locus was mapped to a region similar to the Cq4 locus. On the other hand, the Tgq2 locus overlapped with the QTL region responsible for glucose intolerance (Giq1) that was identified in a previous study. The results suggest that a different combination of QTLs is involved in the trait when a different counterpart strain is used. Identification of distinct, but related traits in an identical chromosomal region will facilitate revealing the responsible gene.
先前对C57BL/6J×KK-Ay/a F2小鼠高脂血症进行的数量性状基因座（QTL）研究确定了三个显著的胆固醇QTL（1号染色体上的Cq1和Cq2，以及3号染色体上的Cq3），以及9号染色体上一个提示性的甘油三酯QTL。该研究的另一项分析在9号染色体上确定了一个新的胆固醇QTL（Cq4），以及9号染色体上一个显著的甘油三酯QTL（Tgq1）。在本研究中，对KK×RR F2小鼠进行了QTL分析。在9号染色体上确定了一个显著的胆固醇QTL（Cq5，LOD值为5.6），在8号染色体上确定了一个显著的甘油三酯QTL（Tgq2，LOD值为4.7）。Cq5基因座被定位到与Cq4基因座相似的区域。另一方面，Tgq2基因座与先前研究中确定的负责葡萄糖不耐受的QTL区域（Giq1）重叠。结果表明，当使用不同的配对品系时，不同的QTL组合参与了该性状。在相同染色体区域鉴定出不同但相关的性状将有助于揭示相关基因。
