Goud S N, Kaplan A M, Subbarao B
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536.
Arch Toxicol. 1992;66(3):164-9. doi: 10.1007/BF01974009.
The influence of cigarette smoke on the humoral immune response of mice was investigated in lymphocytes derived from the spleen, bone marrow (BM) and mesenteric lymph nodes (MLN). Mice of the DBA/2J or C57BL/6 strain were exposed to cigarette smoke of a standard research cigarette, 2R1, twice a day, ten puffs each in morning and afternoon for 20, 40 or 60 weeks. At the end of the smoking period, animals were immunized intraperitoneally with the thymic independent antigens polyvinyl pyrrolidone (PVP) or trinitrophenyl (TNP)-Ficoll. The antibody responses were analyzed using sheep red blood cells coated with PVP or TNP, in a plaque forming cell (PFC) assay. The results indicate a statistically significant inhibition of the antibody response induced by PVP but not by TNP-Ficoll in splenic B cells of smoke exposed mice compared to sham controls. When tested in other lymphoid organs, there was higher anti-TNP PFC response from the BM and MLN cells of smoke exposed animals compared to sham controls.
在来自脾脏、骨髓（BM）和肠系膜淋巴结（MLN）的淋巴细胞中，研究了香烟烟雾对小鼠体液免疫反应的影响。将DBA/2J或C57BL/6品系的小鼠每天两次暴露于标准研究香烟2R1的烟雾中，上午和下午各吸10口，持续20、40或60周。在吸烟期结束时，用胸腺非依赖性抗原聚乙烯吡咯烷酮（PVP）或三硝基苯基（TNP）-Ficoll对动物进行腹腔免疫。在空斑形成细胞（PFC）试验中，使用包被有PVP或TNP的绵羊红细胞分析抗体反应。结果表明，与假对照组相比，暴露于烟雾的小鼠脾脏B细胞中，PVP诱导的抗体反应受到统计学上显著的抑制，但TNP-Ficoll诱导的抗体反应未受抑制。当在其他淋巴器官中进行测试时，与假对照组相比，暴露于烟雾的动物的骨髓和肠系膜淋巴结细胞产生的抗TNP PFC反应更高。
