Williams M J, Hamlin G P, Nimmo A J, Crane L H
Discipline of Physiology and Pharmacology, School of Biomedical Sciences, James Cook University, Townsville, Qld, Australia.
Reprod Fertil Dev. 2003;15(6):311-6. doi: 10.1071/RD03021.
This study compared the nature and magnitude of the contractile response produced in vitro by selective NK1, NK2 and NK3 tachykinin receptor agonists in circularly and longitudinally oriented strips of myometrium from ovariectomised and ovariectomised oestrogen-treated rats. The nature of the responses produced upon stimulation of the tachykinin receptors varied between the different myometrial preparations and the hormonal environment from which the tissue was taken. Variations included: (i) sustained contraction until washout of agonist; (ii) biphasic contraction until washout of agonist; and (iii) monophasic contraction. The major differences in magnitude of contractions were seen in preparations from oestrogen-treated animals in which responses to stimulation of all tachykinin receptors were reduced in comparison to preparations from non-oestrogen treated animals. Furthermore, the responses in circularly oriented myometrium preparations from oestrogen-treated animals were all markedly reduced compared to responses in longitudinally oriented myometrium preparations. These results suggest that the tachykinin receptors in longitudinally and circularly oriented myometrial layers are differentially regulated, especially in tissue isolated from an oestrogen-dominated environment.
本研究比较了选择性NK1、NK2和NK3速激肽受体激动剂在去卵巢大鼠和去卵巢并用雌激素处理的大鼠子宫肌层环形和纵向肌条上体外产生的收缩反应的性质和强度。速激肽受体受刺激后产生的反应性质在不同的子宫肌层制剂以及取材的激素环境之间存在差异。差异包括：（i）持续收缩直至激动剂被洗脱；（ii）双相收缩直至激动剂被洗脱；以及（iii）单相收缩。收缩强度的主要差异见于用雌激素处理的动物的制剂中，与未用雌激素处理的动物的制剂相比，所有速激肽受体受刺激后的反应均降低。此外，与纵向子宫肌层制剂中的反应相比，用雌激素处理的动物的环形子宫肌层制剂中的反应均明显降低。这些结果表明，纵向和环形子宫肌层中的速激肽受体受到不同的调节，尤其是在从雌激素占主导的环境中分离出的组织中。
