通过Toll样受体7和8对单链RNA进行物种特异性识别。

Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8.

作者信息

Heil Florian, Hemmi Hiroaki, Hochrein Hubertus, Ampenberger Franziska, Kirschning Carsten, Akira Shizuo, Lipford Grayson, Wagner Hermann, Bauer Stefan

机构信息

Institute of Medical Microbiology, Immunology and Hygiene, Technische Universität München, Trogerstr. 9, D - 81675 Munich, Germany.

出版信息

Science. 2004 Mar 5;303(5663):1526-9. doi: 10.1126/science.1093620. Epub 2004 Feb 19.

DOI:10.1126/science.1093620

PMID:14976262

Abstract

Double-stranded ribonucleic acid (dsRNA) serves as a danger signal associated with viral infection and leads to stimulation of innate immune cells. In contrast, the immunostimulatory potential of single-stranded RNA (ssRNA) is poorly understood and innate immune receptors for ssRNA are unknown. We report that guanosine (G)- and uridine (U)-rich ssRNA oligonucleotides derived from human immunodeficiency virus-1 (HIV-1) stimulate dendritic cells (DC) and macrophages to secrete interferon-alpha and proinflammatory, as well as regulatory, cytokines. By using Toll-like receptor (TLR)-deficient mice and genetic complementation, we show that murine TLR7 and human TLR8 mediate species-specific recognition of GU-rich ssRNA. These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8.

摘要

双链核糖核酸（dsRNA）作为与病毒感染相关的危险信号，可刺激先天免疫细胞。相比之下，单链RNA（ssRNA）的免疫刺激潜力了解甚少，且尚未发现识别ssRNA的先天免疫受体。我们报告称，源自人类免疫缺陷病毒1型（HIV-1）的富含鸟苷（G）和尿苷（U）的ssRNA寡核苷酸可刺激树突状细胞（DC）和巨噬细胞分泌α干扰素以及促炎和调节性细胞因子。通过使用缺乏Toll样受体（TLR）的小鼠和基因互补技术，我们发现小鼠TLR7和人类TLR8介导对富含GU的ssRNA的物种特异性识别。这些数据表明，ssRNA是TLR7和TLR8的生理性配体。