Stewart Carleton C, Stewart Sigrid J
Laboratory of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, NY, USA.
Methods Mol Biol. 2004;263:45-66. doi: 10.1385/1-59259-773-4:045.
For data acquisition, each supplier provides the software necessary and unique to its instrument. For data analysis, the same software may be used. In addition, several second party vendors provide software often with more capabilities than that provided by the instrument companies. Because of the increase in multiparameter data acquisition, we describe one method for validating instrument performance prior to data acquisition. The fundamentals of data analysis leading to a generic strategy for analysis of any number of parameters are described. This generic approach is designed to simplify the increasing complexity of multiparameter data analysis.