Incorporation into planar lipid bilayers of ATP-regulated K+ channels from membranes of the insulin secreting beta-cell line, HIT T15.

Ion channels were incorporated into planar lipid bilayers following fusion of vesicles from the membrane of an insulin-secreting beta-cell line, HIT T15. The channel was completely blocked by 0.5 mM ATP. The channel retained the same ATP-dependence, voltage-sensitivity and single channel conductance as the ATP-regulated K+ channel that found in isolated membrane patches.