Pruitt Steven C, Mielnicki Lawrence M, Stewart Carleton C
Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY, USA.
Methods Mol Biol. 2004;263:239-58. doi: 10.1385/1-59259-773-4:239.
The process for transfection of cells with expression and gene-trap vectors expressing fluorescent reporter proteins is described. The measurement and sorting of discrete populations of transfected cells is also described and illustrated. Of particular importance, the maintenance of stability may be important and a simple strategy to monitor this has been developed. Finally, an effective method for improving the ability to measure low-level fluorescence from autofluorescence is described.
本文描述了用表达荧光报告蛋白的表达载体和基因捕获载体转染细胞的过程。还介绍并举例说明了对转染细胞离散群体的测量和分选。特别重要的是，维持稳定性可能很重要，并且已经开发出一种简单的策略来对此进行监测。最后，描述了一种提高从自发荧光中测量低水平荧光能力的有效方法。
