Kapoor Veena, Telford William G
Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2004;263:385-98. doi: 10.1385/1-59259-773-4:385.
Telomere length is an important measure of cellular differentiation and progression to senescence. Flow cytometric assays for measuring telomere length have become an important adjunct to more laborious Southern blotting methods; telomere length can be estimated with considerable accuracy in small numbers of individual cells by flow cytometry, and can be measured in cell population subsets with simultaneous fluorescent immunophenotyping. In this chapter, we describe the standard flow cytometric assay for measuring telomere length, including the incorporation of fluorochrome-conjugated antibody immunolabeling for measurement in cell subsets.
端粒长度是细胞分化和衰老进程的一项重要指标。用于测量端粒长度的流式细胞术检测已成为更为繁琐的Southern印迹法的重要辅助手段；通过流式细胞术可以在少量单个细胞中相当准确地估计端粒长度，并且可以在进行同步荧光免疫表型分析的细胞群体亚群中进行测量。在本章中，我们描述了用于测量端粒长度的标准流式细胞术检测方法，包括使用荧光染料偶联抗体免疫标记来对细胞亚群进行测量。
