Paillard F, Sterkers G, Vaquero C
INSERM U152, Institut Cochin de Génétique Moléculaire, Paris, France.
Biochem Biophys Res Commun. 1992 Jul 31;186(2):603-11. doi: 10.1016/0006-291x(92)90789-n.
Full activation of different T cell populations via the TcR/CD3 complex leads to transient expression of lymphokine mRNA and to transient and specific down-modulation of TcR, CD4, CD8 and p56lck mRNA (Multi-Receptor Complex or MRC mRNA). This transient down-modulation is due to both a decrease in transcription and stability of these mRNA and is temporally and quantitatively related to lymphokine mRNA induction. We showed that cyclosporin A (CsA), which blocks lymphokine expression also inhibits MRC mRNA down-modulation at the transcriptional level, and does not affect mRNA stability. The fact that CsA inhibits both lymphokine expression and MRC mRNA down-modulation at transcriptional level supports a model in which similar signals trigger the inverse regulation of these two sets of genes via identical transcriptional factors.
通过TcR/CD3复合物对不同T细胞群体的完全激活会导致淋巴因子mRNA的瞬时表达，以及TcR、CD4、CD8和p56lck mRNA（多受体复合物或MRC mRNA）的瞬时和特异性下调。这种瞬时下调是由于这些mRNA的转录和稳定性降低，并且在时间和数量上与淋巴因子mRNA的诱导相关。我们发现，阻断淋巴因子表达的环孢素A（CsA）在转录水平上也抑制MRC mRNA的下调，并且不影响mRNA的稳定性。CsA在转录水平上同时抑制淋巴因子表达和MRC mRNA下调这一事实支持了一种模型，即相似的信号通过相同的转录因子触发这两组基因的反向调节。
