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Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome.

作者信息

Boldyreff B, Wehr K, Hecht R, Issinger O G

机构信息

Institut für Humangenetik, Universität des Saarlandes, Homburg, Germany.

出版信息

Biochem Biophys Res Commun. 1992 Jul 31;186(2):723-30. doi: 10.1016/0006-291x(92)90806-v.

DOI:10.1016/0006-291x(92)90806-v
PMID:1497660
Abstract

Using the coding region of the human CK-2 alpha cDNA as a probe for screening a genomic mouse library, positive clones representing four different genomic loci were isolated. Partial DNA sequences of these loci encompassing the first 120 nucleotides of the putative coding region are reported. One positive clone was further analyzed by sequencing a 3.1 kb XbaI fragment. This clone displays the characteristics of a pseudogene, i.e. lack of introns and several nucleotide insertions and deletions. In its 3' region it contains a 91 bp large CT-rich stretch which consists of (CCTT) and (CT) repeats; in the 5' region three (CCCCCT) repeats.

摘要

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