Finquelievich J L, Iovannitti C A, Mujica M T, Relloso S, Carnovale S, de Elías Costa M R, Jewtuchowicz V, Espinel-Ingroff A
Center of Mycology, Department of Microbiology, Parasitology and Immunology, School of Medicine, Paraguay 2155, 1121 Buenos Aires, Argentina.
Rev Argent Microbiol. 2003 Oct-Dec;35(4):214-8.
In vitro susceptibilities of 290 isolates of Candida spp to fluconazole were evaluated by a new NCCLS M44-P agar disk diffusion method that was read and interpreted automatically by the BIOMIC image-analysis plate reader system. Disk test results were compared to results obtained by a modified NCCLS M27-A broth microdilution method using RPMI-1640 supplemented with 2% dextrose. Overall agreement between both methods was 90.0%. Category agreement between the broth and disk test results for susceptible, susceptible dose-dependent and resistant disk results were 99.6%, 19.05% and 52.17%, respectively. No very major discrepancies, 1.03% major discrepancies, and 8.97% minor discrepancies were observed between results of the two test methods. This analysis suggests that results from both methods correlate highly for Candida strains susceptible to fluconazole. The lower agreement between the two methods for resistant and susceptible dose-dependent isolates was due to strains near or on that breakpoint, different media (Mueller-Hinton vs RPMI), agar vs broth, 80% vs 50% endpoints, and trailing growth near the endpoints.
采用一种新的NCCLS M44-P琼脂纸片扩散法对290株念珠菌属菌株进行氟康唑体外药敏试验，该方法由BIOMIC图像分析平板读数仪系统自动读取和判读。将纸片试验结果与采用添加2%葡萄糖的RPMI-1640培养基的改良NCCLS M27-A肉汤微量稀释法所获结果进行比较。两种方法的总体一致性为90.0%。肉汤法和纸片法药敏试验结果对于敏感、剂量依赖性敏感和耐药纸片结果的类别一致性分别为99.6%、19.05%和52.17%。两种试验方法结果之间未观察到极重大差异，重大差异为1.03%，微小差异为8.97%。该分析表明，对于对氟康唑敏感的念珠菌菌株，两种方法的结果高度相关。两种方法对耐药和剂量依赖性敏感菌株的一致性较低，原因在于接近或处于该折点的菌株、不同培养基（Muller-Hinton与RPMI）、琼脂与肉汤、80%与50%终点以及终点附近的拖尾生长。
