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糙皮侧耳对松针的降解作用

[Degradation of pine needles by Stereum hirsutum].

作者信息

Mouso N, Diorio L A, Forchiassin F

机构信息

Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, Piso 4, 1428 Buenos Aires, Argentina.

出版信息

Rev Argent Microbiol. 2003 Oct-Dec;35(4):219-23.

Abstract

Pine-needle degradation by Stereum hirsutum was studied under conditions of solid state fermentation with the aim of accelerating its decomposition, avoiding the accumulation in situ and in view of the possible utilization of the residual organic matter. Three experimental systems were tested: pine needles alone and with the addition of either a nitrogen source or barley grain. Determinations were made at 14 and 28 days of incubation. All treatments showed substrate degradation. The addition of a nitrogen source raised enzymatic activities measured but not the degree of degradation. Grain addition resulted in higher biomass, enzyme activities, sugar accumulation and degradation of the substrate. Fungal biomass estimated as N-acetyl glucosamine allowed calculation of the actual degradation of the substrate, that reached 19% at 28 d of culture without additions and 44% at 14 d in pine-needles with grain.

摘要

为了加速松针分解、避免其在原地积累,并考虑到残余有机物的可能利用,研究了毛革盖菌在固态发酵条件下对松针的降解情况。测试了三个实验系统:单独的松针以及添加了氮源或大麦粒的松针。在培养14天和28天时进行测定。所有处理均显示出底物降解。添加氮源提高了所测的酶活性,但未提高降解程度。添加谷物导致更高的生物量、酶活性、糖积累和底物降解。以N-乙酰葡糖胺估算的真菌生物量可用于计算底物的实际降解情况,在无添加物的培养28天时底物降解率达到19%,在添加谷物的松针中培养14天时降解率达到44%。

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