Levin Laura, Diorio Luis, Grassi Emanuel, Forchiassin Flavia
Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, PROPLAME-PHRIDEB, CONICET Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, Piso 4, (1428) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Apr-Jun;44(2):105-12.
The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxidase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58% for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xylidine, respectively, in 5 h.
本研究旨在评估农业工业废弃物葡萄茎杆通过固态发酵，利用以下三种白腐真菌：栓菌属真菌、毛革盖菌和南极革盖菌进行生长和木质纤维素酶生产的潜力。还研究了上述培养物对几种染料的脱色情况。60天后测得底物干重损失值相近（33%-43%）。南极革盖菌产生的漆酶和锰过氧化物酶活性最高（分别为33.0和1.6 U/g干固体）。毛革盖菌培养物中内切葡聚糖酶产量最高（10.4 U/g），而木聚糖内切酶活性峰值出现在栓菌属真菌中（14.6 U/g）。南极革盖菌/葡萄茎杆体系在纺织加工废水的生物修复方面似乎具有潜在竞争力，在5小时内对靛蓝胭脂红、孔雀石绿、天青B、活性艳蓝R、结晶紫和二甲苯胺的脱色率分别达到93%、86%、82%、82%、77%和58%。
