Nowatzki Timothy M, Niimi Bradly, Warren Kelli J, Putnam Sean, Meinke Lance J, Gosselin David C, Harvey F Edwin, Hunt Thomas E, Siegfried Blair D
Department of Entomology, University of Nebraska-Lincoln, 202 Plant Industry Bldg., Lincoln, NE 68583-0816, USA.
J Econ Entomol. 2003 Dec;96(6):1750-9. doi: 10.1093/jee/96.6.1750.
Field and laboratory studies were conducted in 2000 and 2001 to determine the feasibility of mass marking western corn rootworm adults, Diabrotica virgifera virgifera LeConte, with RbCl in the field. Results showed that application of rubidium (Rb) in solution to both the soil (1 g Rb/plant) and whorl (1 g Rb/plant) of corn plants was optimal for labeling western corn rootworm adults during larval development. Development of larvae on Rb-enriched corn with this technique did not significantly influence adult dry weight or survival. Rb was also highly mobile in the plant. Application of Rb to both the soil and the whorl resulted in median Rb concentrations in the roots (5,860 ppm) that were 150-fold greater than concentrations in untreated roots (38 ppm) 5 wk after treatment. Additionally, at least 90% of the beetles that emerged during the first 3 wk were labeled above the baseline Rb concentration (5 ppm dry weight) determined from untreated beetles. Because emergence was 72% complete at this time, a significant proportion of the population had been labeled. Results from laboratory experiments showed that labeled beetles remained distinguishable from unlabeled beetles for up to 4 d postemergence. The ability to efficiently label large numbers of beetles under field conditions and for a defined period with virtually no disruption of the population provides an unparalleled opportunity to conduct mark-recapture experiments for quantifying the short-range, intrafield movement of adult corn rootworms.
2000年和2001年开展了田间和实验室研究，以确定在田间用氯化铷对西部玉米根虫成虫（Diabrotica virgifera virgifera LeConte）进行大规模标记的可行性。结果表明，在玉米植株的土壤（每株1克铷）和叶心（每株1克铷）中施用铷溶液，对于在幼虫发育期间标记西部玉米根虫成虫而言是最佳的。采用这种技术在富含铷的玉米上饲养幼虫，对成虫的干重或存活率没有显著影响。铷在植株内的移动性也很强。在处理后5周，在土壤和叶心都施用铷，使根中的铷浓度中位数（5860 ppm）比未处理根中的浓度（38 ppm）高150倍。此外，在前3周羽化的甲虫中，至少90%的甲虫的铷浓度高于从未处理甲虫中确定的基线铷浓度（干重5 ppm）。由于此时羽化完成了72%，所以相当大比例的种群已被标记。实验室实验结果表明，标记后的甲虫在羽化后长达4天仍可与未标记的甲虫区分开来。在田间条件下能够有效地标记大量甲虫，并在规定时期内几乎不干扰种群，这为进行标记重捕实验以量化成年玉米根虫的短距离田间内移动提供了无与伦比的机会。
