Chai Young-Mi, Lim Bu-Kug, Lee Jong-Yoon, Kim Mu-Nam, Park Mo-Ra, Rhee Soon-Jae
Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongbuk, Korea.
J Med Food. 2003 Winter;6(4):329-36. doi: 10.1089/109662003772519886.
This study investigated the effect of a manufactured soluble dietary fiber on lipid metabolism in rats fed high cholesterol diets. Soluble dietary fiber was prepared from wood chips of oak (Quercus mongolica). Male Sprague-Dawley rats weighing 100 +/- 10 g were randomly assigned to either a normal diet or five high cholesterol diets containing 1% cholesterol and different fiber supplements. The high cholesterol groups were subdivided into fiber-free diet (FF), 5% pectin (5P), 10% pectin (10P), 5% manufactured soluble dietary fiber (5QM), and 10% manufactured soluble dietary fiber (10QM) groups. Total serum cholesterol concentrations in all soluble dietary fiber-supplemented groups were lower than in the FF group. The high-density lipoprotein-cholesterol concentration in the FF group was significantly lower, compared with the normal group, but was increased in groups supplemented with soluble dietary fiber. Low-density lipoprotein-cholesterol levels and the atherogenic index had the same tendency as total cholesterol concentration. Compared with the FF group, in the 5P, 5QM, 10P, and 10QM groups hepatic triglyceride concentrations were 12%, 16%, 20%, and 24% lower, respectively, and hepatic cholesterol concentrations were 48%, 52%, 52%, and 58% lower, respectively. Hepatic 3-hydroxy-3-methylglutaryl-CoA reductase activity in the soluble fiber groups was significantly higher than in the FF groups, but lower than the normal group. When hepatic tissue was observed under a light microscope, the FF group had completely formed lipomas in the hepatic tissue, which led to fat deposits and then a fatty liver. The size and number of lipomas were lower in the soluble dietary fiber-fed groups, as compared with the group not fed dietary fiber. In conclusion, improvements in lipid metabolism were observed as a result of the manufactured soluble dietary fiber from the oak chips, and were similar to that seen for pectin. The preparation method for the soluble dietary fiber from oak chips successfully produced a functional soluble fiber.
本研究调查了一种人工合成的可溶性膳食纤维对喂食高胆固醇饮食大鼠脂质代谢的影响。可溶性膳食纤维由蒙古栎木片制备而成。将体重为100±10克的雄性斯普拉格-道利大鼠随机分为正常饮食组或五种含1%胆固醇及不同纤维补充剂的高胆固醇饮食组。高胆固醇组再细分为无纤维饮食（FF）组、5%果胶（5P）组、10%果胶（10P）组、5%人工合成可溶性膳食纤维（5QM）组和10%人工合成可溶性膳食纤维（10QM）组。所有补充可溶性膳食纤维组的总血清胆固醇浓度均低于FF组。FF组的高密度脂蛋白胆固醇浓度与正常组相比显著降低，但在补充可溶性膳食纤维的组中有所升高。低密度脂蛋白胆固醇水平和动脉粥样硬化指数与总胆固醇浓度具有相同趋势。与FF组相比，5P、5QM、10P和10QM组的肝脏甘油三酯浓度分别降低了12%、16%、20%和24%，肝脏胆固醇浓度分别降低了48%、52%、52%和58%。可溶性纤维组的肝脏3-羟基-3-甲基戊二酰辅酶A还原酶活性显著高于FF组，但低于正常组。在光学显微镜下观察肝组织时，FF组在肝组织中完全形成了脂肪瘤，导致脂肪沉积进而形成脂肪肝。与未喂食膳食纤维的组相比，喂食可溶性膳食纤维的组中脂肪瘤的大小和数量较少。总之，观察到由栎木片制成的人工合成可溶性膳食纤维改善了脂质代谢，且与果胶的效果相似。从栎木片制备可溶性膳食纤维的方法成功生产出了一种功能性可溶性纤维。
