Park Y B, Do K M, Bok S H, Lee M K, Jeong T S, Choi M S
Department of Genetic Engineering, Kyungpook National University, Taegu, 702-701, Korea.
Int J Vitam Nutr Res. 2001 Jan;71(1):36-44. doi: 10.1024/0300-9831.71.1.36.
Certain bioflavonoids are potent antioxidants and have pharmacologic effects similar to those of vitamin E. Accordingly, the interactive effect of hesperidin and vitamin E was studied with respect to cholesterol metabolism and the antioxidant status. Hesperidin supplement (0.1%, wt/wt) with comparable levels of vitamin E was provided with a high-cholesterol (1%, wt/wt) diet to rats for 5 weeks. The amount of vitamin E included in the hesperidin-free and hesperidin diets was either a low (low-E) or a normal (normal-E) level. The hesperidin supplement and different levels of dietary vitamin E did not significantly alter the concentrations of plasma triglycerides. However, the inclusion of hesperidin significantly lowered the concentration of plasma cholesterol in both the low-vitamin E group and the normal-vitamin E group compared to the hesperidin-free groups (p < 0.05). The hepatic triglyceride content was significantly lowered by the hesperidin supplement, as opposed to the plasma triglyceride content, regardless of the vitamin E level in the diet. The hepatic HMG-CoA reductase activity was significantly lowered by the hesperidin supplement with both the low-vitamin E and the normal-vitamin E compared to the hesperidin-free groups (p < 0.05). The hepatic HMG-CoA reductase activity was also significantly lowered with an increase in the dietary vitamin E within the hesperidin and hesperidin-free groups. The excretion of fecal neutral sterol and acidic sterols tended to be lower with the hesperidin supplement. Neither dietary hesperidin nor vitamin E significantly changed the hepatic antioxidant enzyme activity. This data indicates that hesperidin lowers the concentration of plasma cholesterol and the hepatic triglyceride content regardless of the dietary vitamin E level. However, the concentration of plasma cholesterol in the hesperidin-free groups was dependent on the dietary vitamin E level. This information may contribute to understanding the interactive effect of hesperidin and vitamin E on cholesterol biosynthesis in high cholesterol-fed rats.
某些生物类黄酮是有效的抗氧化剂，具有与维生素E相似的药理作用。因此，研究了橙皮苷和维生素E在胆固醇代谢和抗氧化状态方面的相互作用。将含有可比水平维生素E的橙皮苷补充剂（0.1%，重量/重量）与高胆固醇（1%，重量/重量）饮食一起提供给大鼠5周。不含橙皮苷和含橙皮苷饮食中维生素E的含量分别为低水平（低E）或正常水平（正常E）。橙皮苷补充剂和不同水平的膳食维生素E并未显著改变血浆甘油三酯的浓度。然而，与不含橙皮苷的组相比，在低维生素E组和正常维生素E组中，添加橙皮苷均显著降低了血浆胆固醇的浓度（p < 0.05）。与血浆甘油三酯含量不同，无论饮食中维生素E水平如何，橙皮苷补充剂均显著降低了肝脏甘油三酯含量。与不含橙皮苷的组相比，低维生素E和正常维生素E的橙皮苷补充剂均显著降低了肝脏HMG-CoA还原酶活性（p < 0.05）。在含橙皮苷和不含橙皮苷的组中，随着膳食维生素E的增加，肝脏HMG-CoA还原酶活性也显著降低。添加橙皮苷后，粪便中性固醇和酸性固醇的排泄量有降低趋势。膳食中的橙皮苷和维生素E均未显著改变肝脏抗氧化酶活性。这些数据表明，无论膳食维生素E水平如何，橙皮苷均可降低血浆胆固醇浓度和肝脏甘油三酯含量。然而，不含橙皮苷组的血浆胆固醇浓度取决于膳食维生素E水平。这些信息可能有助于理解橙皮苷和维生素E对高胆固醇喂养大鼠胆固醇生物合成的相互作用。
