Fang Baijun, Shi Mingxia, Liao Lianming, Yang Shaoguang, Liu Yuhao, Zhao Robert Chunhua
Sino-American Collaborative Laboratory, State Key Laboratory of Experimental Haematology, Institute of Haematology and Blood Diseases Hospital, and Tissue Engineering Center, Chinese Academy of Medical Sciences, Tianjin 300020, China.
J Hematother Stem Cell Res. 2003 Dec;12(6):603-13. doi: 10.1089/15258160360732632.
We previously reported that Flk1(+)/CD31(-)/CD34(-) cells isolated from human fetal bone marrow can differentiate at the single cell level into endothelial and hematopoietic cells in vitro. Here we report that within this cell population reside cells that can differentiate into the epithelium of liver, lung, gut, as well as the cells of both hematopoietic and endothelial system after primary or secondary transplantation into irradiated nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Hence, Flk1(+)/CD31(-)/CD34(-) cells possess remarkable differentiation potential and may thereby provide an alternative to hematopoietic stem cells for transplantation. In addition, our results show this stem cell population effectively accelerated wound healing in NOD/SCID mice and thus holds therapeutic promise for treatment of genetic disorders, organ dysfunction, and tissue repair in humans.
我们之前报道过，从人胎骨髓中分离出的Flk1(+)/CD31(-)/CD34(-)细胞在体外单细胞水平可分化为内皮细胞和造血细胞。在此我们报道，在这个细胞群体中存在一些细胞，将其初次或二次移植到经照射的非肥胖糖尿病/重症联合免疫缺陷（NOD/SCID）小鼠体内后，它们能分化为肝、肺、肠道上皮细胞以及造血和内皮系统的细胞。因此，Flk1(+)/CD31(-)/CD34(-)细胞具有显著的分化潜能，从而可能为造血干细胞移植提供一种替代方案。此外，我们的结果表明，这个干细胞群体能有效加速NOD/SCID小鼠的伤口愈合，因此对治疗人类的遗传疾病、器官功能障碍和组织修复具有治疗前景。
