Kautzky M, Susani M, Schenk P
II. Universitäts-Hals-Nasen-Ohren-Klinik Wien.
Laryngorhinootologie. 1992 Jul;71(7):347-52. doi: 10.1055/s-2007-997312.
The in-vivo tissue ablation characteristics of a pulsed infrared laser (Holmium:YAG, lambda = 1980) and a pulsed excimer laser (XeCl, lambda = 308 nm) were studied. The holmium laser energy was coupled into nylon fibres. The excimer laser energy was coupled into quartz fibers. Laser incisions were made on white rats at different sites of the tongue. Power settings of 400 mJ per 2.5 microseconds pulse at 5 pulses per second for the holmium:YAG laser and 250 mJ per 1 microsecond pulse at 20 pulses per second for the excimer laser were used. Thermal gradients following laser application were measured. Excellent control of depth of tissue ablation was noted for both types of lasers. The tissue response was examined by light microscopy. Histological studies revealed tissue defects with clean contours. The coagulative necrosis zone for the 400 mJ holmium:YAG injuries measured from 370 microns to 640 microns, compared with 40 microns to 160 microns with the 250 mJ UV-excimer injuries. Wound healing was studied over a six-week period. Histologically, wound closure was completed after four weeks showing re-epithelialization without scar formation for both types of laser. The in-vivo tissue ablation characteristics and the operation reliability of the two lasers were compared.
研究了脉冲红外激光（钬激光：波长1980nm）和脉冲准分子激光（XeCl，波长308nm）的体内组织消融特性。钬激光能量耦合到尼龙纤维中。准分子激光能量耦合到石英纤维中。在白色大鼠舌头的不同部位进行激光切口。钬激光的功率设置为每2.5微秒脉冲400mJ，每秒5个脉冲；准分子激光的功率设置为每1微秒脉冲250mJ，每秒20个脉冲。测量激光照射后的热梯度。两种类型的激光对组织消融深度均有良好的控制。通过光学显微镜检查组织反应。组织学研究显示组织缺损轮廓清晰。400mJ钬激光损伤的凝固性坏死区为370微米至640微米，而250mJ紫外线准分子激光损伤的凝固性坏死区为40微米至160微米。在六周的时间内研究伤口愈合情况。组织学上，四周后伤口闭合完成，两种类型的激光均显示重新上皮化且无瘢痕形成。比较了两种激光的体内组织消融特性和操作可靠性。
