Jung Chul Won, Kwon Jung Hye, Seol Jae Goo, Park Woo Hyun, Hyun Jung Mi, Kim Eun Shil, Kim Seung Taik, Lee Sang Jae, Kim Byoung Kook, Lee Young Yiul
Division of Hematology/Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Am J Hematol. 2004 Mar;75(3):121-7. doi: 10.1002/ajh.10471.
Peptide-pulsed dendritic cells can stimulate T cells showing specific cytotoxicity in chronic myelogenous leukemia. We tried to induce a specific cytotoxic T-cell response stimulated by RNA-pulsed dendritic cells in acute myelogenous leukemia. The total RNA of WEHI-3BD+, a myelomonocytic leukemia cell line derived from BALB/c mice, was transfected into dendritic cells induced from bone marrow nucleated cells of BALB/c mice with granulocyte macrophage colony-stimulating factor (GM-CSF) and lipopolysaccharide (LPS) using liposome. RNA-pulsed dendritic cells were injected into the peritoneal cavity of BALB/c mice, and splenic T cells were isolated for antigen-stimulated proliferation and leukemia-specific cytotoxicity assay. Cultured bone marrow nucleated cells expressed dendritic cell markers including MHC class II antigen, CD80, CD86, and CD11c. T cells stimulated by RNA-pulsed dendritic cells showed enhanced proliferation than those stimulated by unpulsed dendritic cells (P = 0.05) and showed dose-dependent specific cytotoxicity against WEHI-3BD+ cells. We concluded total RNA-pulsed dendritic cells could induce a specific T-cell cytotoxicity in acute myelogenous leukemia.
肽脉冲树突状细胞可刺激慢性粒细胞白血病中表现出特异性细胞毒性的T细胞。我们试图在急性髓细胞白血病中诱导由RNA脉冲树突状细胞刺激的特异性细胞毒性T细胞反应。使用脂质体将源自BALB/c小鼠的髓单核细胞白血病细胞系WEHI-3BD+的总RNA转染到用粒细胞巨噬细胞集落刺激因子（GM-CSF）和脂多糖（LPS）从BALB/c小鼠骨髓有核细胞诱导而来的树突状细胞中。将RNA脉冲树突状细胞注入BALB/c小鼠的腹腔，并分离脾T细胞用于抗原刺激增殖和白血病特异性细胞毒性测定。培养的骨髓有核细胞表达包括MHC II类抗原、CD80、CD86和CD11c在内的树突状细胞标志物。与未脉冲树突状细胞刺激的T细胞相比，RNA脉冲树突状细胞刺激的T细胞增殖增强（P = 0.05），并且对WEHI-3BD+细胞表现出剂量依赖性特异性细胞毒性。我们得出结论，总RNA脉冲树突状细胞可在急性髓细胞白血病中诱导特异性T细胞细胞毒性。
