牛体外受精胚胎的形态发育与性别

Morphological development and sex of bovine in vitro-fertilized embryos.

作者信息

Avery B, Jørgensen C B, Madison V, Greve T

机构信息

Department of Clinical Studies, Royal Veterinary and Agricultural University, Copenhagen, Denmark.

出版信息

Mol Reprod Dev. 1992 Jul;32(3):265-70. doi: 10.1002/mrd.1080320312.

DOI:10.1002/mrd.1080320312

PMID:1497876

Abstract

Bovine in vitro-fertilized embryos at the blastocyst stage were collected at days 7, 8, and 10 postinsemination and sex was determined via the polymerase chain reaction (PCR) to compare the embryonic development with the sex of the embryos. The percentages of males (sex ratio) after division of the embryos into three developmental groups were 68%, 48%, and 35% in the fast, intermediate, and slow groups, respectively (P = 0.014). The percentages of males on days 7, 8, and 10 were 60%, 40% and 33%, respectively (P = 0.043). The average sex ratio for the whole material was 50%. It is thus concluded that male bovine preimplantation embryos develop faster than female embryos.

摘要

在授精后第7天、第8天和第10天收集处于囊胚期的牛体外受精胚胎，并通过聚合酶链反应（PCR）确定胚胎性别，以比较胚胎发育与胚胎性别的关系。将胚胎分为三个发育组后，快速发育组、中等发育组和缓慢发育组的雄性胚胎百分比（性别比例）分别为68%、48%和35%（P = 0.014）。第7天、第8天和第10天的雄性胚胎百分比分别为60%、40%和33%（P = 0.043）。整个样本的平均性别比例为50%。因此得出结论，雄性牛植入前胚胎比雌性胚胎发育得更快。