Wang Zhaoming, Lai Fernand Mac-Moune
Department of Pathology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310003, China.
Zhonghua Nan Ke Xue. 2004 Jan;10(1):26-8, 31.
To detect the status of loss of heterozygosity (LOH) on chromosome 8 in prostate carcinoma and high grade prostatic intraepithelial neoplasia (PIN).
Pure DNA was obtained from prostate neoplasms and normal tissues by tissue microdissection. LOH on chromosome 8 was detected by PCR based microsatellite polymorphism analysis technique using 14 pairs of microsatellite primers in 10 samples of prostate carcinoma and 10 samples of high grade PIN.
There were different frequencies of LOH on chromosome 8 in 10 samples of prostate carcinoma. 8p23.1-p23.2 and 8p21-p22 were two high-frequency LOH regions. LOH on chromosome 8 was detected in 3 samples of high grade PIN.
There were high-frequency LOH regions on chromosome 8 of prostate carcinoma, located on 8p23.1-p23.2 and 8p21-p22. High grade PIN and prostate carcinoma share the same allelic loss on 8p. Tumor suppressor genes located at these two regions may be potentially involved in the initiation and progression of prostate carcinoma.
检测前列腺癌及高级别前列腺上皮内瘤变（PIN）中8号染色体杂合性缺失（LOH）的情况。
通过组织显微切割从前列腺肿瘤及正常组织中获取纯净DNA。采用基于聚合酶链反应（PCR）的微卫星多态性分析技术，使用14对微卫星引物，对10例前列腺癌样本和10例高级别PIN样本进行8号染色体LOH检测。
10例前列腺癌样本中8号染色体LOH频率各异。8p23.1 - p23.2和8p21 - p22是两个高频LOH区域。在3例高级别PIN样本中检测到8号染色体LOH。
前列腺癌8号染色体存在高频LOH区域，位于8p23.1 - p23.2和8p21 - p22。高级别PIN和前列腺癌在8p存在相同的等位基因缺失。位于这两个区域的肿瘤抑制基因可能潜在参与前列腺癌的发生和发展。
