Xu Ming, Li Shiwen, Zheng Xinmin, Hu Liquan
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Zhonghua Nan Ke Xue. 2004 Jan;10(1):39-41.
To study the relationship between the apoptosis and the expression of inducible nitric oxide synthase (iNOS) and Bcl-2 in prostate carcinoma (PCa).
Expression of Bcl-2 and iNOS and apoptotic cells in 24 cases of PCa, 15 cases of BPH and 5 cases of normal prostate tissues were detected by immunohistochemical technique and terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), respectively.
Apoptosis index (AI) and iNOS-positive index of PCa were much higher than that of the benign prostate hyperplasia (BPH) and the normal prostate group(P < 0.01). The AI of the Bcl-2-positive group was lower than that of the negative ones in PCa(P < 0.01).
AI might serve as a marker in evaluating the aggression of PCa. The iNOS-positive index of PCa had no relationship with the differentiated grades but had a negative relationship with the expression of Bcl-2. The expression of Bcl-2 protein was negatively related to PCa cell apoptosis. Both iNOS and Bcl-2 were believed to play roles in the pathogenesis and development of PCa by influencing the cell apoptosis.
研究前列腺癌（PCa）中细胞凋亡与诱导型一氧化氮合酶（iNOS）及Bcl-2表达之间的关系。
分别采用免疫组织化学技术和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测24例PCa、15例良性前列腺增生（BPH）及5例正常前列腺组织中Bcl-2、iNOS的表达及凋亡细胞情况。
PCa的凋亡指数（AI）和iNOS阳性指数显著高于良性前列腺增生（BPH）组和正常前列腺组（P<0.01）。PCa中Bcl-2阳性组的AI低于阴性组（P<0.01）。
AI可作为评估PCa侵袭性的指标。PCa的iNOS阳性指数与分化程度无关，但与Bcl-2的表达呈负相关。Bcl-2蛋白表达与PCa细胞凋亡呈负相关。iNOS和Bcl-2均被认为通过影响细胞凋亡在PCa的发病机制和发展过程中发挥作用。
