Chappell John S, Meyn Ashleigh W, Ngim Kenley K
Drug Enforcement Administration, Western Laboratory, San Francisco, CA 94105, USA.
J Forensic Sci. 2004 Jan;49(1):52-9.
The chemical analysis of gamma-hydroxybutyric acid (GHB) in most forensic laboratories is complicated by the highly polar nature of the GHB molecule, which makes it unsuitable for direct analysis by gas chromatography (GC). Consequently, a popular analytical approach is to convert GHB into the corresponding lactone or a derivative compound that is then identified by mass spectrometry employed in conjunction with GC (GC/MS). An alternative approach is presented here where GHB may be isolated as a free acid specie from complex aqueous solutions employing a liquid-liquid extraction technique. This approach can yield a relatively pure residue of GHB that presents an infrared transmission spectrum that is sufficiently distinct for identification purposes. Infrared spectroscopy (IR) is a very popular technique that is available to most crime laboratories. The liquid-liquid extraction behavior of GHB is examined in detail and the uniqueness of the infrared spectrum is discussed.
在大多数法医实验室中，γ-羟基丁酸（GHB）的化学分析因GHB分子的高极性而变得复杂，这使得它不适用于气相色谱法（GC）直接分析。因此，一种常用的分析方法是将GHB转化为相应的内酯或衍生化合物，然后通过与GC联用的质谱法（GC/MS）进行鉴定。本文提出了另一种方法，即采用液-液萃取技术从复杂的水溶液中分离出游离酸形式的GHB。这种方法可以得到相对纯净的GHB残留物，其红外透射光谱具有足够的独特性以供鉴定之用。红外光谱法（IR）是大多数犯罪实验室都能使用的一种非常常用的技术。本文详细研究了GHB的液-液萃取行为，并讨论了红外光谱的独特性。
