采用二极管阵列检测液相色谱法测定尿液和水基质中的除虫菊酯和拟除虫菊酯类农药。
Determination of pyrethrin and pyrethroid pesticides in urine and water matrixes by liquid chromatography with diode array detection.
作者信息
Loper Bobby L, Anderson Kim A
机构信息
Oregon State University, Department of Environmental and Molecular Toxicology, Food Safety and Environmental Stewardship Program, 1007 Agriculture and Life Sciences Building, Corvallis, OR 97331, USA.
出版信息
J AOAC Int. 2003 Nov-Dec;86(6):1236-40.
The following pyrethrin and pyrethroid pesticides were determined in urine and water matrixes by liquid chromatography with diode array detection (LC-DAD): pyrethrin I, pyrethrin II, tetramethrin, baythroid, bifenthrin, fenvalerate, phenothrin, allethrin, resmethrin, cis-permethrin, and trans-permethrin. In addition, 3-phenoxybenzyl alcohol, a metabolite of various pyrethroids, was also successfully determined by the analytical method. The matrix extraction was simple, inexpensive, and fast, using only sodium chloride and acetonitrile. The acetonitrile extract was filtered and analyzed by LC-DAD. The method detection limits for the pyrethrin pesticides in 5 mL urine were determined to range from 0.002 to 0.04 microg/mL, depending on the individual pyrethrin. Recoveries from spiked tap water ranged from 77 to 96%; recoveries from urine ranged from 80 to 117%. This method is especially well-suited to clinical investigations, in which rapid analysis of forensic samples is often required.
采用液相色谱 - 二极管阵列检测法（LC - DAD）对尿液和水基质中的以下除虫菊酯和拟除虫菊酯类农药进行了测定：除虫菊酯 I、除虫菊酯 II、胺菊酯、倍硫磷、联苯菊酯、氰戊菊酯、苯醚菊酯、丙烯菊酯、炔咪菊酯、顺式氯菊酯和反式氯菊酯。此外，还通过该分析方法成功测定了多种拟除虫菊酯类农药的代谢产物 3 - 苯氧基苄醇。基质萃取过程简单、成本低廉且快速，仅使用氯化钠和乙腈。乙腈萃取液经过过滤后用 LC - DAD 进行分析。5 mL 尿液中除虫菊酯类农药的方法检出限根据具体的除虫菊酯而定，范围为 0.002 至 0.04 μg/mL。加标自来水的回收率为 77%至 96%；尿液的回收率为 80%至 117%。该方法特别适用于临床研究，因为临床研究中常常需要对法医样本进行快速分析。
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