沉积在聚对苯二甲酸乙二酯上的非晶碳膜对细菌黏附的影响。

The effects of amorphous carbon films deposited on polyethylene terephthalate on bacterial adhesion.

作者信息

Wang J, Huang N, Yang P, Leng Y X, Sun H, Liu Z Y, Chu P K

机构信息

School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

出版信息

Biomaterials. 2004 Jul;25(16):3163-70. doi: 10.1016/j.biomaterials.2003.10.010.

DOI:10.1016/j.biomaterials.2003.10.010

PMID:14980411

Abstract

There is an increasing interest in developing new methods to reduce bacteria adhesion onto polymeric materials that are used in biomedical implants. The antibacterial behavior on polyethylene terephthalate (PET) treated by acetylene (C2H2) plasma immersion ion implantation-deposition (PIII-D) is investigated. The surface structure of the treated PET is determined by laser Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). The results show that a thin amorphous polymer-like carbon (PLC) layer is formed on the PET surface. Atomic force micrographs (AFM) show that C2H2 PIII-D significantly changes the surface morphology of PET. The capacities of Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE) to adhere onto PET are quantitatively determined by plate counting and Gamma-ray counting of 125I radio labeled bacteria in vitro. The results indicate that the adhesion of the two kinds of bacteria to PET is suppressed by PLC. The adhesion efficiency of SE on the coated surface is only about 14% of that of the untreated PET surface, and that of SA is about 35% of that of the virgin surface. The electrokinetic potentials of the bacterial cells and substrates are determined by zeta potential measurement. All the substrates as well as the bacterial strain have negative zeta potentials, and it means that bacterial adhesion is not mediated by electrostatic interactions. The surface energy components of the various substrates and bacteria are calculated based on measurements in water, formamide and diiodomethane. The surface free energies obtained are used to calculate the interfacial free energies of adhesion ( deltaFAdh ) of SA and SE onto various substrates, and it is found that bacterial adhesion is energetically unfavorable on the PLC deposited on PET by C2H2 PIII-D.

摘要

开发新方法以减少细菌在生物医学植入物所用聚合物材料上的黏附，正受到越来越多的关注。本文研究了通过乙炔（C2H2）等离子体浸没离子注入沉积（PIII-D）处理的聚对苯二甲酸乙二酯（PET）的抗菌行为。通过激光拉曼光谱、X射线光电子能谱（XPS）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）确定了处理后PET的表面结构。结果表明，PET表面形成了一层薄的类非晶聚合物碳（PLC）层。原子力显微镜图像（AFM）显示，C2H2 PIII-D显著改变了PET的表面形态。通过平板计数和体外125I放射性标记细菌的伽马射线计数，定量测定了金黄色葡萄球菌（SA）和表皮葡萄球菌（SE）在PET上的黏附能力。结果表明，PLC抑制了两种细菌对PET的黏附。SE在涂层表面的黏附效率仅为未处理PET表面的约14%，SA的黏附效率约为原始表面的35%。通过zeta电位测量确定了细菌细胞和底物的电动电位。所有底物以及细菌菌株的zeta电位均为负，这意味着细菌黏附不是由静电相互作用介导的。基于在水、甲酰胺和二碘甲烷中的测量结果，计算了各种底物和细菌的表面能成分。所获得的表面自由能用于计算SA和SE在各种底物上的黏附界面自由能（deltaFAdh），结果发现，对于通过C2H2 PIII-D沉积在PET上的PLC，细菌黏附在能量上是不利的。