Shresta Sujan, Kyle Jennifer L, Robert Beatty P, Harris Eva
Division of Infectious Diseases, School of Public Health, University of California at Berkeley, Berkeley, CA 94720-7360, USA.
Virology. 2004 Feb 20;319(2):262-73. doi: 10.1016/j.virol.2003.09.048.
Dengue virus (DEN) causes the most prevalent arthropod-borne viral illness in humans worldwide. Immune mechanisms that are involved in protection and pathogenesis of DEN infection have not been fully elucidated due largely to the lack of an adequate animal model. Therefore, as a first step, we characterized the primary immune response in immunocompetent inbred A/J mice that were infected intravenously with a non-mouse-adapted DEN type 2 (DEN2) strain. A subset (55%) of infected mice developed paralysis by 14 days post-infection (p.i.), harbored infectious DEN in the central nervous system (CNS), and had an elevated hematocrit and a decreased white blood cell (WBC) count. Immunologic studies detected (i). increased numbers of CD69(+) splenic natural killer (NK) and B cells at day 3 p.i., (ii). DEN-specific IgM and IgG responses by days 3 and 7 p.i., respectively, and (iii). splenocyte production of IFNgamma at day 14 p.i. We conclude that the early activities of NK cells, B cells and IgM, and later actions of IFNgamma and IgG likely play a role in the defense against DEN infection.
登革病毒（DEN）引发全球人类中最普遍的节肢动物传播病毒性疾病。由于缺乏合适的动物模型，参与登革病毒感染的保护和发病机制的免疫机制尚未完全阐明。因此，作为第一步，我们对具有免疫活性的近交系A/J小鼠进行了特征描述，这些小鼠通过静脉注射非小鼠适应的2型登革病毒（DEN2）株进行感染。一部分（55%）受感染小鼠在感染后14天（p.i.）出现麻痹，中枢神经系统（CNS）中存在传染性登革病毒，血细胞比容升高，白细胞（WBC）计数降低。免疫学研究检测到：（i）感染后第3天脾脏自然杀伤（NK）细胞和B细胞中CD69(+)数量增加；（ii）感染后第3天和第7天分别出现登革病毒特异性IgM和IgG反应；（iii）感染后第14天脾细胞产生IFNγ。我们得出结论，NK细胞、B细胞和IgM的早期活动以及IFNγ和IgG的后期作用可能在抵御登革病毒感染中发挥作用。
