Synthesis of 7'-[123I]iodo-D-luciferin for in vivo studies of firefly luciferase gene expression.

作者信息

Lee Sang-Yoon, Choe Yearn Seong, Lee Kyung-Han, Lee Jeewoo, Choi Yong, Kim Byung-Tae

机构信息

Department of Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-ku, Seoul 135-710, South Korea.

出版信息

Bioorg Med Chem Lett. 2004 Mar 8;14(5):1161-3. doi: 10.1016/j.bmcl.2003.12.066.

DOI:10.1016/j.bmcl.2003.12.066

PMID:14980656

Abstract

D-(-)-2-(6'-hydroxy-7'-[(123)I]iodobenzothiazolyl)-delta(2)-thiazoline-4-caroxylic acid (7'-[(123)I]iodo-D-luciferin) was synthesized as a novel reporter probe for in vivo studies of firefly luciferase gene expression. 7'-Iodo-D-luciferin, a nonradioactive standard, was synthesized and showed the binding property (K(M)=4.28 microM) similar to that of D-luciferin (2.53 microM) for firefly luciferase in luminescence assay.

摘要