[胎儿大鼠唇裂与无瘢痕伤口愈合的宫内模型]
[Intra-uterine model of cleft lip and scarless wound healing of fetal rats].
作者信息
Cui Lei, Zhang Qun, Li Fa-cheng, Qian Yun-liang, Guan Wen-xiang
机构信息
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2002 Sep;11(3):216-8.
OBJECTIVE
To properly evaluate the benefits of in utero repair of cleft lip, an animal model of cleft lip was developed and the fetal scarless wound healing was observed in SD rat fetus.
METHODS
Through a maternal celiotomy and hysterotomy, twenty excisional cleft was created in the upper lip of rat fetus. Another group of 20 rat fetus were given full-thickness incisional wounds in the upper lips. The fetus with cleft lip were returned into utero for further development till birth.Wound specimens from the incisional wounds in the other group were removed successively 12, 24, 48, 72h postwounding for histological examination.
RESULTS
The fetus with excisional wound demonstrated a complete cleft lip after birth. In contrast, the wound in the fetus with incisional wounds regenerated with normal architecture histologically, indistinguishable from the surrounding normal skin.
CONCLUSION
The results of this preliminary study indicates that the fetal rat cleft lip is technically feasible with an excellent survival rate. Healing occurs without scar formation. This model will be used to document facial growth following in utero repair of a cleft lip.
目的
为了正确评估唇裂宫内修复的益处，建立了唇裂动物模型，并在SD大鼠胎儿中观察了胎儿无瘢痕伤口愈合情况。
方法
通过母体剖腹术和子宫切开术，在大鼠胎儿的上唇制造20个切除性唇裂。另一组20只大鼠胎儿的上唇给予全层切开伤口。唇裂胎儿返回子宫进一步发育直至出生。另一组切开伤口的伤口标本在受伤后12、24、48、72小时依次取出进行组织学检查。
结果
有切除伤口的胎儿出生后表现为完全性唇裂。相比之下，有切开伤口的胎儿的伤口在组织学上以正常结构再生，与周围正常皮肤无明显差异。
结论
这项初步研究结果表明，胎儿大鼠唇裂修复在技术上是可行的，存活率很高。愈合过程中无瘢痕形成。该模型将用于记录唇裂宫内修复后的面部生长情况。
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