Robinson B W, Goss A N
Cleft Palate J. 1981 Oct;18(4):251-5.
Cheek wounds, involving skin, mucosa, and muscle, were surgically created in utero on Sprague Dawley rat fetuses at 19 1/2 days gestation. Fifty fetuses were used in the study. Twenty-five fetuses were in the experimental group and 25 in the control. Five fetuses in each group were sacrificed at 0, 6, 12, 18 and 24 hours post-operatively. Serial histological sections in the coronal plane were stained with haematoxylin and eosin. Restoration of continuity and primary epithelialisation of the oral mucosal and skin surfaces of the wound was observed to occur within 24 hours. The continuity of the muscle layer was not restored, but some mitoses were seen in the myeloblasts. There was a complete absence of acute inflammatory cells, and there was no scab formation. Healing occurred without scarring. These findings are discussed in comparison to previous findings on orofacial fetal wound healing (Goss 1976) and in regard to future prospects in intraceptive fetal surgery for the correction of deformities, in particular cleft lip.
在妊娠19.5天的斯普拉格-道利大鼠胎儿子宫内通过手术造成涉及皮肤、黏膜和肌肉的脸颊伤口。该研究使用了50只胎儿。25只胎儿为实验组，25只为对照组。每组分别在术后0、6、12、18和24小时处死5只胎儿。冠状面的连续组织学切片用苏木精和伊红染色。观察到伤口的口腔黏膜和皮肤表面在24小时内恢复连续性并初步上皮化。肌肉层的连续性未恢复，但在成肌细胞中可见一些有丝分裂。完全没有急性炎症细胞，也没有结痂形成。愈合过程中无瘢痕形成。将这些发现与先前关于胎儿口腔面部伤口愈合的研究结果（戈斯，1976年）进行了比较，并探讨了未来在胎儿期进行手术矫正畸形（特别是唇裂）的前景。
