Yokel R A, Allen D D, Burgio D E, McNamara P J
College of Pharmacy, University of Kentucky, Lexington.
J Pharmacol Toxicol Methods. 1992 May;27(3):135-42. doi: 10.1016/1056-8719(92)90034-x.
Antipyrine was investigated as a dialyzable substance that could be used to quantitate relative differences in the efficiency of dialysis among multiple microdialysis probes and by a single probe over time. The contribution of effective membrane surface area to recovery variability was tested by the introduction of air into microdialysis probes. Reduction of effective membrane surface area reduced antipyrine recovery. Dialysates from probes implanted in the jugular vein, brain, and liver of rats receiving antipyrine demonstrated differences in antipyrine concentration among probes within the same rat. These results suggest dissimilar efficiencies of the probes to recover antipyrine, which should be uniformly distributed throughout body water. Dialysates from blood, brain, and liver probes in rats that received both antipyrine and tritiated water (3H2O) showed differences in antipyrine and 3H2O concentrations among probes. Variability of antipyrine and 3H2O concentrations over time within a probe were positively correlated, suggesting that the cause(s) of temporal variability affected both of these markers of body water. Correction of antipyrine tissue/blood ratios, using 3H2O blood/tissue ratios from the same sampling period, reduced the variability in antipyrine tissue/blood ratios, producing ratios closer to the expected value of 1. Differences in probe efficiency contributing to the variability of antipyrine and 3H2O recovery would also be expected to influence the recovery of other substances during microdialysis. The administration of antipyrine during microdialysis experiments is suggested to enable reduction of temporal and site-related differences in substance recovery that are due to differences in probe efficiency. Other methods are necessary to determine the actual extracellular concentration of dialyzed substances and the integrity of the blood-brain barrier.
安替比林作为一种可透析物质进行了研究，它可用于定量多个微透析探针之间以及单个探针随时间变化的透析效率的相对差异。通过向微透析探针中引入空气来测试有效膜表面积对回收率变异性的影响。有效膜表面积的减小会降低安替比林的回收率。从接受安替比林的大鼠的颈静脉、脑和肝脏中植入的探针获得的透析液显示，同一大鼠体内不同探针的安替比林浓度存在差异。这些结果表明，探针回收安替比林的效率不同，而安替比林应均匀分布于全身水分中。接受安替比林和氚标记水（3H2O）的大鼠的血液、脑和肝脏探针的透析液显示，不同探针的安替比林和3H2O浓度存在差异。探针内安替比林和3H2O浓度随时间的变异性呈正相关，这表明时间变异性的原因影响了这两种身体水分标志物。使用同一采样期的3H2O血液/组织比值校正安替比林组织/血液比值，可降低安替比林组织/血液比值的变异性，使比值更接近预期值1。预计导致安替比林和3H2O回收率变异性的探针效率差异也会影响微透析过程中其他物质的回收率。建议在微透析实验期间给予安替比林，以减少由于探针效率差异导致的物质回收的时间和部位相关差异。还需要其他方法来确定透析物质的实际细胞外浓度和血脑屏障的完整性。
