Yusta-Boyo María J, González Manuel A, Pavón Nancy, Martín Ana B, De La Fuente Ricardo, García-Castro Javier, De Pablo Flora, Moratalla Rosario, Bernad Antonio, Vicario-Abejón Carlos
Group of Growth Factors in Vertebrate Development, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Ramiro de Maeztu 9, E-28040 Madrid, Spain.
Eur J Neurosci. 2004 Feb;19(3):505-12. doi: 10.1111/j.1460-9568.2004.03140.x.
Neural stem cells giving rise to neurons and glia cells have been isolated from the embryonic and adult central nervous system. The extent to which they are able to differentiate into cells of non-neural lineages, such as the hematopoietic lineage, is nonetheless unclear. We previously reported the isolation of stem cells from the mouse olfactory bulb neuroepithelium. In the present study, we analysed whether olfactory bulb stem cells (OBSC) can generate cells with hematopoietic features. Cells were prepared from the olfactory bulbs of transgenic mice expressing enhanced green fluorescent protein (EGFP). In culture, transgenic cells proliferated with the same kinetics as wild-type cells. Following mitogen removal, both cell types gave rise to similar numbers of neurons, astrocytes and oligodendrocytes, indicating that EGFP overexpression does not alter OBSC proliferation and differentiation patterns. When these cells were injected into the tail vein of irradiated mice, no hematopoietic cells derived from the OBSC could be recovered in their peripheral blood, spleen or bone marrow. By contrast, when OBSC were transplanted into the adult brain, EGFP-positive cells were found in the striatum and corpus callosum; differentiated cells expressed antigenic markers of neurons and astrocytes. These results suggest that embryonic olfactory bulb stem cells are not endowed with the potential to produce hematopoiesis.
已从胚胎和成年中枢神经系统中分离出能产生神经元和神经胶质细胞的神经干细胞。然而，它们能够分化为非神经谱系细胞（如造血谱系细胞）的程度尚不清楚。我们之前报道了从小鼠嗅球神经上皮中分离出干细胞。在本研究中，我们分析了嗅球干细胞（OBSC）是否能产生具有造血特征的细胞。细胞取自表达增强型绿色荧光蛋白（EGFP）的转基因小鼠的嗅球。在培养过程中，转基因细胞与野生型细胞以相同的动力学增殖。去除促有丝分裂原后，两种细胞类型产生的神经元、星形胶质细胞和少突胶质细胞数量相似，这表明EGFP的过度表达不会改变OBSC的增殖和分化模式。当将这些细胞注入受辐照小鼠的尾静脉时，在其外周血、脾脏或骨髓中未发现源自OBSC的造血细胞。相比之下，当将OBSC移植到成年大脑中时，在纹状体和胼胝体中发现了EGFP阳性细胞；分化细胞表达神经元和星形胶质细胞的抗原标志物。这些结果表明，胚胎嗅球干细胞不具备产生造血作用的潜能。
