Gartner A, Castellon W T, Gallon G, Simondon F
UR 4F, Département Santé, ORSTOM (l'Institut Français de Recherche Scientifique pour le Développement en Coopération), Montpellier, France.
Nutrition. 1992 Jul-Aug;8(4):258-65.
To study the immunological effect of total dietary restriction, BALB/c mice were limited to reduced body growth or maintained at practically constant body weight between 21 and 61 days of age by giving them 70% (R70%) or 55% (R55%) of the average daily food intake of the control group fed ad libitum. Thymus and spleen weight were decreased, but thymus size was maintained in proportion to body weight in R70% mice, whereas the ratio of thymus weight to body weight was significantly decreased in R55% mice. In restricted mice, splenocytes showed a lower percentage of B lymphocytes and a higher percentage of T lymphocytes. Results of stimulation showed that proliferation capacity was increased for B lymphocytes and decreased for T lymphocytes in restricted mice. Our data show the importance of studying the thymus over a period, because normal thymus growth was first greatly impaired by dietary restriction, and subsequent thymus involution was delayed, showing that an isolated comparison between the thymus of growing mice is not enough. Our data show the interest of determining the percentage of lymphocyte populations parallel to their response to mitogen stimulation.
为研究完全饮食限制的免疫效应，在21至61日龄期间，通过给予BALB/c小鼠对照组随意进食平均每日食物摄入量的70%（R70%）或55%（R55%），使其身体生长受限或维持体重基本恒定。胸腺和脾脏重量减轻，但R70%小鼠的胸腺大小与体重成比例维持，而R55%小鼠的胸腺重量与体重之比显著降低。在饮食受限的小鼠中，脾细胞显示B淋巴细胞百分比降低，T淋巴细胞百分比升高。刺激结果表明，饮食受限小鼠的B淋巴细胞增殖能力增强，T淋巴细胞增殖能力降低。我们的数据表明，长期研究胸腺很重要，因为正常胸腺生长首先因饮食限制而严重受损，随后胸腺退化延迟，这表明仅比较生长中小鼠的胸腺是不够的。我们的数据表明，确定淋巴细胞群体百分比及其对有丝分裂原刺激反应的相关性很有意义。
