Parra Adalberto, Reyes-Terán Gustavo, Ramírez-Peredo Jorge, Jacquemin Benedicte, Quiroz Verónica, Cárdenas Mario, García-Sancho María Cecilia, Larrea Fernando
Department of Endocrinology, Instituto Nacional de Perinatología, México City, Mexico.
Immunol Cell Biol. 2004 Feb;82(1):24-31. doi: 10.1111/j.1440-1711.2004.01201.x.
The existence of decreased hypothalamic dopaminergic tone in HIV-infected men has been suggested. In a cross-sectional study, we determined 12 h nocturnal basal and pulsatile prolactin (PRL) release levels (by blood sampling every 10 min) and their correlation with CD4+ T cells in seven volunteer HIV-negative, healthy men (group 1), and 21 normoprolactinemic, euthyroid, HIV-infected men divided into 3 groups (each group = 7): (i) group 2, asymptomatic HIV-infected stage A1 men, untreated; (ii) group 3, AIDS stage C3 without active opportunistic infections, untreated; and (iii) group 4, previously stage C3 after at least 6 months of successful highly active antiretroviral therapy. Serum PRL was measured by radioimmunoanalysis and the results were analysed by waveform-independent deconvolution analysis. CD4+ T lymphocytes were measured by flow cytometry and viral load by a nucleic acid sequence-based amplification assay. No differences were detected in the first two groups. In the third group, however, 100% of prolactin secretion was found to be pulsatile with a shorter secretory burst duration (P = 0.04), and a greater circulating half-life and pulse amplitude (P < or = 0.04). Group 4 had the greatest basal prolactin secretion (P < or = 0.04), and a shorter secretory burst duration (P = 0.04 vs group 2), circulating half-life (P = 0.01 vs group 3) and intersecretory burst interval (P = 0.06 vs group 1). PRL approximate entropy was similar among all groups. Linear correlations existed between CD4+ T cell counts and PRL secretory burst half duration (r = 0.62, P = 0.002) and amplitude (r = -0.63, P = 0.001), and in circulating serum half-life (r = - 0.61, P = 0.002) in HIV-infected groups. Viral load showed no correlations. It is suggested that differential changes in nocturnal prolactin secretion among HIV-infected men occurred while maintaining the normal coordinate feedback and/or feedforward control within the lactotropic axis. These changes may represent an adaptative mechanism to sustain, by different means, the maximal physiologic PRL production to stimulate the highest cellular immune response and/or reconstitution in attempting to survive.
有研究表明，感染HIV的男性下丘脑多巴胺能张力降低。在一项横断面研究中，我们测定了7名HIV阴性的健康男性志愿者（第1组）以及21名催乳素正常、甲状腺功能正常的HIV感染男性（分为3组，每组7人）的12小时夜间基础及脉冲性催乳素（PRL）释放水平（每10分钟采血一次），并分析了其与CD4+ T细胞的相关性：（i）第2组，无症状HIV感染A1期男性，未接受治疗；（ii）第3组，艾滋病C3期且无活动性机会性感染，未接受治疗；（iii）第4组，曾处于C3期，经过至少6个月成功的高效抗逆转录病毒治疗。采用放射免疫分析法测定血清PRL，并通过与波形无关的反卷积分析法分析结果。通过流式细胞术检测CD4+ T淋巴细胞，采用基于核酸序列扩增的检测方法检测病毒载量。在前两组中未检测到差异。然而，在第3组中，发现100%的催乳素分泌呈脉冲性，分泌脉冲持续时间较短（P = 0.04），循环半衰期和脉冲幅度较大（P≤0.04）。第4组基础催乳素分泌量最大（P≤0.04），分泌脉冲持续时间较短（与第2组相比，P = 0.04），循环半衰期（与第3组相比，P = 0.01）和分泌脉冲间期（与第1组相比，P = 0.06）。所有组的PRL近似熵相似。在HIV感染组中，CD4+ T细胞计数与PRL分泌脉冲半衰期（r = 0.62，P = 0.002）、幅度（r = -0.63，P = 0.001）以及循环血清半衰期（r = -0.61，P = 0.002）之间存在线性相关性。病毒载量未显示相关性。提示HIV感染男性夜间催乳素分泌存在差异变化，同时维持了促乳素轴内正常的协调反馈和/或前馈控制。这些变化可能代表一种适应性机制，通过不同方式维持最大生理性PRL分泌，以刺激最高的细胞免疫反应和/或重建，从而试图存活。
