Liu Dongyou, Ainsworth A Jerald, Austin Frank W, Lawrence Mark L
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, 39762, USA.
Int J Food Microbiol. 2004 Mar 15;91(3):297-304. doi: 10.1016/j.ijfoodmicro.2003.07.004.
Listeria monocytogenes is an opportunistic bacterial pathogen that has accounted for an important portion of human foodborne diseases worldwide. In this study, through comparative analysis of L. innocua and L. monocytogenes genomic sequences, we selected a L. monocytogenes specific gene (lmo0733) that has the potential for specific detection of L. monocytogenes. Using PCR primers (lmo0733F and lmo0733R) derived from this gene, a specific fragment of 453 bp was amplified only from genomic DNA of L. monocytogenes strains. PCR products from other Listeria species as well as other Gram-positive and -negative species were not detectable, confirming the specificity of this assay. Thus, the PCR test employing primers lmo0733F and lmo0733R represents an additional tool in the diagnostic arsenal for rapid, sensitive and specific detection and identification of human infections due to L. monocytogenes.
单核细胞增生李斯特菌是一种机会性细菌病原体，在全球人类食源性疾病中占重要比例。在本研究中，通过对无害李斯特菌和单核细胞增生李斯特菌基因组序列的比较分析，我们选择了一个单核细胞增生李斯特菌特异性基因（lmo0733），该基因具有特异性检测单核细胞增生李斯特菌的潜力。使用源自该基因的PCR引物（lmo0733F和lmo0733R），仅从单核细胞增生李斯特菌菌株的基因组DNA中扩增出一个453 bp的特异性片段。来自其他李斯特菌属物种以及其他革兰氏阳性和阴性物种的PCR产物均未检测到，证实了该检测方法的特异性。因此，采用引物lmo0733F和lmo0733R的PCR检测代表了诊断工具库中的一种额外工具，可用于快速、灵敏和特异性地检测和鉴定由单核细胞增生李斯特菌引起的人类感染。
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